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| Status |
Public on Dec 01, 2012 |
| Title |
Expression data from transgenic Nicotiana tabacum expressing a variant of coat protein and movement protein from Tobacco mosaic virus |
| Platform organism |
Solanum lycopersicum |
| Sample organism |
Nicotiana tabacum |
| Experiment type |
Expression profiling by array
|
| Summary |
Transgenic expression of viral proteins in natural host plants is a useful simplified system with the potential to understand the individual effect of each viral component. Transgenic expression of movement (MP) and a variant from coat protein (CPT42W) in tobacco, a TMV natural host, produces severe morphological changes, altered miRNAs accumulation and poor fertility.
We used microarrays to characterize the gene expression changes caused by the co-expression of TMV capsid and movement proteins in Nicotiana tabacum comparing two isogenic lines MPxCPT42W and mpxcpT42W* (a line with both transgenes spontaneously silenced and with normal phenotype).
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| Overall design |
Leaf tissues from 6-week old tobacco plants MPxCPT42W and mpxcpT42W* were collected for RNA extraction and hybridization on Affymetrix microarrays. We collected pools of three plants (one biological replicate) and analyzed three independent biological replicates for each transgenic line.
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| Contributor(s) |
Conti G, Asurmendi S |
| Citation(s) |
22712510 |
| |
| Submission date |
May 10, 2012 |
| Last update date |
Dec 24, 2015 |
| Contact name |
Gabriela Conti |
| Organization name |
INTA
|
| Street address |
N. repetto y Los Reseros
|
| City |
Buenos Aires |
| ZIP/Postal code |
1686 |
| Country |
Argentina |
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| Platforms (1) |
| GPL4741 |
[Tomato] Affymetrix Tomato Genome Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA165143 |
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