|
| Status |
Public on Dec 01, 2012 |
| Title |
Tobacco CPxMPT42W,rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
tobacco plants expressing MP and CPT42W from TMV
|
| Organism |
Nicotiana tabacum |
| Characteristics |
transgene: expressing MP and CPT42W from TMV
tissue: leaf
cultivar: xanthi
age: 6 wk
|
| Treatment protocol |
Stable transgenic isogenic lines. The first line co-expresses movement protein and a variant of coat protein from Tobacco mosaic virus (TMV) and the second line has sponatneously silenced both transgenes
|
| Growth protocol |
Tobacco plants were grown in greenhouse with 16-8hs photoperiod, temperature ranging from 25-22°C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with RNeasy™ Plant Mini kit (Qiagen), according to manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Gene Chip Expression Analysis Technical Manual, 2004, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Tomato Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Gene Chip Scanner 3000 7G
|
| Description |
Gene expression data from MPxCPT42W
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
May 10, 2012 |
| Last update date |
Dec 01, 2012 |
| Contact name |
Gabriela Conti |
| Organization name |
INTA
|
| Street address |
N. repetto y Los Reseros
|
| City |
Buenos Aires |
| ZIP/Postal code |
1686 |
| Country |
Argentina |
| |
|
| Platform ID |
GPL4741 |
| Series (1) |
| GSE37905 |
Expression data from transgenic Nicotiana tabacum expressing a variant of coat protein and movement protein from Tobacco mosaic virus |
|
