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| Status |
Public on Jan 01, 2014 |
| Title |
Regulated expression of polysaccharide utilization and capsular biosynthesis loci in biofilm and planktonic Bacteroides thetaiotaomicron during growth in chemostats |
| Platform organisms |
Bacteroides thetaiotaomicron; Phocaeicola vulgatus; Parabacteroides distasonis; Methanobrevibacter smithii; Lachnospira eligens; Agathobacter rectalis; Bifidobacterium longum |
| Sample organism |
Bacteroides thetaiotaomicron VPI-5482 |
| Experiment type |
Expression profiling by array
|
| Summary |
We used Affymetrix GeneChips to determine the physiological differences between biofilm and planktonic cells of Bacteroides thetaiotaomicron strain VPI-5482 (ATCC 29148) by comparing gene expression. For this purpose, B. thetaiotaomicron cells were grown in sterile, continuous flow bioreactors fed with tryptone, yeast extract, glucose (TYG) medium. The bioreactors were controlled at a temperature of 37C using a water jacket and a recirculating water heater. After 8 hours post-inoculation, planktonic cells were harvested from the bulk solution in the bioreactor, and after 8 days post-inoculation, the biofilm was scraped from the carbon paper. RNA was harvested from both biofilm and planktonic populations. RNA was extracted by a phenol:chloroform method and purified with a Qiagen RNA Easy mini-kit.
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| Overall design |
Overall growth conditions are summarized above. The experimental conditions were: biofilm (BF), and planktonic (PL).
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| Contributor(s) |
He Z, TerAvest M |
| Citation(s) |
23996813 |
| |
| Submission date |
Jun 06, 2012 |
| Last update date |
Jan 30, 2015 |
| Contact name |
Michaela Anne TerAvest |
| E-mail(s) |
mat273@cornell.edu
|
| Organization name |
Cornell University
|
| Department |
Biological and Environmental Engineering
|
| Lab |
Lars Angenent
|
| Street address |
B45 Riley-Robb Hall
|
| City |
Ithaca |
| State/province |
New York |
| ZIP/Postal code |
14853 |
| Country |
USA |
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| Platforms (2) |
| GPL1821 |
bthetaa511044F |
| GPL7006 |
[BacEubMsa520350F] Affymetrix Human gut microbiota community GeneChip |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA168084 |
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