|
| Status |
Public on Jan 31, 2013 |
| Title |
Identification of ORB1 target genes using inducible overexpression system |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
|
| Summary |
The aim was to identify genes associated to the down-regulation TARGET OF RAPAMYCIN (TOR) pathway in Arabidopsis. For this purpose, three independent amiR-tor lines (amiR-tor9, amiR-tor17 and amiR-tor 20) and EV lines 3 and 6 days after EST- or non-induction were used for expression profile analysis using Affymetrix microarray.
Two weeks-old transgenic seedlings were transferred to MS plate with 20µM estradiol to induce the overexpression of amiR-tor (consequently, repression of AtTOR levels) under the control of an estradiol-(EST) inducible promoter. Identically treated wild-type Col-0 and pER8 empty-vector (EV) transformed seedlings were served as controls. Seedlings were harvested after 3 and 6 (amiR-tor lines) days.
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| |
|
| Overall design |
genetic modification
|
| |
|
| Contributor(s) |
Caldana C, Giavalisco P |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jun 22, 2012 |
| Last update date |
Aug 15, 2018 |
| Contact name |
Camila Caldana |
| E-mail(s) |
camila.caldana@bioetanol.org.br
|
| Phone |
00551935123585
|
| Organization name |
Brazilian Bioethanol Science and Technology Laboratory
|
| Street address |
Rua Giuseppe Máximo Scolfaro, 10.000
|
| City |
Campinas |
| State/province |
Sao Paulo |
| ZIP/Postal code |
13083-970 |
| Country |
Brazil |
| |
|
| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
|
| Samples (16)
|
|
| Relations |
| Affiliated with |
GSE69995 |
| BioProject |
PRJNA169133 |
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