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Status |
Public on Jan 31, 2013 |
Title |
Identification of ORB1 target genes using inducible overexpression system |
Organism |
Arabidopsis thaliana |
Experiment type |
Expression profiling by array
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Summary |
The aim was to identify genes associated to the down-regulation TARGET OF RAPAMYCIN (TOR) pathway in Arabidopsis. For this purpose, three independent amiR-tor lines (amiR-tor9, amiR-tor17 and amiR-tor 20) and EV lines 3 and 6 days after EST- or non-induction were used for expression profile analysis using Affymetrix microarray. Two weeks-old transgenic seedlings were transferred to MS plate with 20µM estradiol to induce the overexpression of amiR-tor (consequently, repression of AtTOR levels) under the control of an estradiol-(EST) inducible promoter. Identically treated wild-type Col-0 and pER8 empty-vector (EV) transformed seedlings were served as controls. Seedlings were harvested after 3 and 6 (amiR-tor lines) days.
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Overall design |
genetic modification
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Contributor(s) |
Caldana C, Giavalisco P |
Citation missing |
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Submission date |
Jun 22, 2012 |
Last update date |
Aug 15, 2018 |
Contact name |
Camila Caldana |
E-mail(s) |
camila.caldana@bioetanol.org.br
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Phone |
00551935123585
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Organization name |
Brazilian Bioethanol Science and Technology Laboratory
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Street address |
Rua Giuseppe Máximo Scolfaro, 10.000
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City |
Campinas |
State/province |
Sao Paulo |
ZIP/Postal code |
13083-970 |
Country |
Brazil |
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Platforms (1) |
GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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Samples (16)
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Relations |
Affiliated with |
GSE69995 |
BioProject |
PRJNA169133 |