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Series GSE40088 Query DataSets for GSE40088
Status Public on Aug 14, 2012
Title Comparative transcriptomic analysis of acute host responses during 2009 pandemic H1N1 influenza infection in mouse, macaque, and swine (macaque dataset)
Platform organism Macaca mulatta
Sample organism Macaca fascicularis
Experiment type Expression profiling by array
Summary Background: The 2009 pandemic H1N1 influenza virus emerged in swine and quickly became a major global health threat. In mouse, non-human primate, and swine infection models, the pH1N1 virus efficiently replicates in the lung and induces pro-inflammatory host responses; however, whether similar or different cellular pathways were impacted by pH1N1 virus across independent infection models remains to be further defined. To address this, we have performed a comparative transcriptomic analysis of acute host responses to a single pH1N1 influenza virus, A/California/04/2009 (CA04), in the lung of mice, macaques and swine.

Results: Despite similarities in the clinical course, we observed differences in inflammatory molecules elicited, and the kinetics of their gene expression changes across all three species. The retinoid X receptor (RXR) signaling pathway controlling pro-inflammatory and metabolic processes was differentially regulated during infection in each species, though the heterodimeric RXR partner, pathway associated signaling molecules, and gene expression patterns differed in each species.

Conclusions: By comparing transcriptional changes in the context of clinical and virological measures, we identified differences in the host transcriptional response to pH1N1 virus across independent models of acute infection. Antiviral resistance and the emergence of new influenza viruses have placed more focus on developing drugs that target the immune system. Underlying overt clinical disease are molecular events that suggest therapeutic targets identified in one host may not be appropriate in another.
 
Overall design The goal of this experiment was to use global gene expression profiling to understand non-human primate lung cellular responses to pandemic H1N1 influenza A/California/04/2009 virus infection. Four- to fifteen-year-old cynomologous macaques were infected with CA04 virus (n = 4) under anesthesia through a combination of intratracheal (4 ml), intranasal (0.5 ml per nostril), conjunctival (0.5 ml per eyelid) and oral (1 ml) routes with a suspension containing 10^6 TCID50/ml (total infectious dose was 7x10^6 TCID50). Animals were euthanized on 1 and 6 days post-inoculation (n = 2 per time point), and total RNA was extracted from lung samples and analyzed by microarray. Pooled RNA from lungs of uninfected animals served as the reference.
 
Contributor(s) Tisoncik Go J, Belisle SE, Tchitchek N, Tumpey TM, Ma W, Richt JA, Feldmann H, Katze MG
Citation(s) 23153050
Submission date Aug 13, 2012
Last update date Aug 12, 2017
Contact name Michael Katze
E-mail(s) data@viromics.washington.edu
Organization name University of Washington
Department Microbiology
Lab Michael G. Katze, Ph.D
Street address Rosen Building 960 Republican St.
City Seattle
State/province WA
ZIP/Postal code 98109-4325
Country USA
 
Platforms (1)
GPL14569 Agilent-015421 Rhesus Macaque Gene Expression Microarray (Probe Name Version)
Samples (6)
GSM984814 H1N1_Exp1_141M_a_CA_LL_Day6
GSM984815 H1N1_Exp1_143M_b_CA_LL_Day1
GSM984816 H1N1_Exp1_Battelle_rep1
This SubSeries is part of SuperSeries:
GSE40092 Comparative transcriptomic analysis of acute host responses during 2009 pandemic H1N1 influenza infection in mouse, macaque, and swine
Relations
BioProject PRJNA172764

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Supplementary file Size Download File type/resource
GSE40088_RAW.tar 41.3 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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