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| Status |
Public on Aug 21, 2012 |
| Title |
Expression data of AI4 CD8 T cells from AI4 and Rip-B7xAI4 mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
B7x (B7-H4 or B7S1) is the seventh member of the B7 family and the in vivo function remains largely unknown. Despite new genetic data linking the B7x gene with autoimmune diseases, how exactly it contributes to peripheral tolerance and autoimmunity is unclear. Here we showed that B7x protein was not detected on antigen-presenting cells or T cells in both human and mice, which is unique in the B7 family. As B7x protein is expressed in some peripheral cells such as pancreatic b cells, we utilized a CD8 T cell-mediated diabetes model (AI4ab) in which CD8 T cells recognize an endogenous self-antigen, and found that mice lacking B7x developed more severe diabetes than control AI4ab mice. Conversely, mice overexpressing B7x in the b cells (Rip-B7xAI4ab) were diabetes free. Furthermore, adoptive transfer of effector AI4ab CD8 T cells induced diabetes in control mice, but not in Rip-B7xAI4ab mice. Mechanistic studies revealed that pathogenic effector CD8 T cells were capable of migrating to the pancreas but failed to robustly destroy tissue when encountering local B7x in Rip-B7xAI4ab mice. Although AI4ab CD8 T cells in Rip-B7xAI4ab mice and AI4ab mice showed similar cytotoxic function, cell death, and global gene expression profiles, these cells had greater proliferation in AI4ab mice than in RIP-B7xAI4ab mice. These results suggest that B7x in nonlymphoid organs prevents peripheral autoimmunity partially through inhibiting proliferation of tissue-specific CD8 T cells and that local overexpression of B7x on pancreatic b cells is sufficient to abolish CD8 T cell-induced diabetes.
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| Overall design |
AI4 mice developed T1D and Rip-B7x AI4 mice were resistant to T1D.
Total RNA was collected and gene expression compared between AI4 CD8 T cells from AI4 and Rip-B7xAI4 mice.
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| Contributor(s) |
Zang X, Lee JS |
| Citation(s) |
22972920 |
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| Submission date |
Aug 20, 2012 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Juan Lin |
| Organization name |
Albert Einstein College of Medicine
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| Street address |
1300 Morris Park Ave.
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| City |
Bronx |
| State/province |
NY |
| ZIP/Postal code |
10461 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| GSM988585 |
AI4 from AI4 mice, biological replicate 1 |
| GSM988586 |
AI4 from AI4 mice, biological replicate 2 |
| GSM988587 |
AI4 from AI4 mice, biological replicate 3 |
| GSM988589 |
AI4 from Rip-B7xAI4 mice, biological replicate 1 |
| GSM988590 |
AI4 from Rip-B7xAI4 mice, biological replicate 2 |
| GSM988591 |
AI4 from Rip-B7xAI4 mice, biological replicate 3 |
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| Relations |
| BioProject |
PRJNA173237 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE40225_RAW.tar |
23.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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