|
| Status |
Public on Aug 21, 2012 |
| Title |
AI4 from AI4 mice, biological replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
AI4 from AI4 mice
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6.H2g7/g7 background
genotype/variation: AI4
disease status: developed T1D
tissue: pancreas
cell type: AI4αβ CD8 T cells
|
| Growth protocol |
Single-cell suspensions of pancreata from AI4 mice or Rip-B7xAI4 mice were pooled and AI4αβ CD8 T cells were isolated by FACS sorting using anti-CD8 Ab and MimA2/H-2Db tetramer
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified according to manufacturrer's instructions using Qiagen Rnesway kit
|
| Label |
biotin
|
| Label protocol |
Samples were labeled according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Samples were hybridized according to the standard Affymetrix protocol
|
| Scan protocol |
Gene ST 1.0 chips were scanned using Affymetrix 7G
|
| Description |
Gene expression data
Sample name: XZ.PAN.U.8717
|
| Data processing |
Data were analyzed using Affymetrix GenChip operating software 1.4 and the quality tested using the Affymetrix Expression Console.
All positive, negative and spike-in control probe sets and probe sets without mRNA Description information were not included in the analysis.
|
| |
|
| Submission date |
Aug 20, 2012 |
| Last update date |
Aug 21, 2012 |
| Contact name |
Juan Lin |
| Organization name |
Albert Einstein College of Medicine
|
| Street address |
1300 Morris Park Ave.
|
| City |
Bronx |
| State/province |
NY |
| ZIP/Postal code |
10461 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE40225 |
Expression data of AI4 CD8 T cells from AI4 and Rip-B7xAI4 mice |
|
