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| Status |
Public on Oct 23, 2012 |
| Title |
Androgen receptor splice variants mediate enzalutamide resistance in castration-resistant prostate cancer cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The goal of this experiment was to compare the gene expression programs mediated by androgen/AR vs. constitutively active, truncated AR variants in castration-resistant CWR-R1 prostate cancer cells. Because constitutive activity of truncated AR variants can mask androgen/AR target genes, the androgen/AR transcriptional program was assessed by silencing the trucnated AR 1/2/3/CE3 variant with siRNA targeting AR exon CE3 and treating cells with vehicle (ethanol) or 1nM DHT. Similarly, because full-length AR activity can mask truncated AR variant target genes, the AR variant transcriptional program was assessed under castrate conditions by selectively silencing full-length AR with siRNA targeting AR exon 7, and comparing this profile with CWR-R1 cells transfected wtih siRNA targeting AR exon 1, which silences all AR expression (full-length and truncated AR variants).
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| Overall design |
CWR-R1 cells were maintained under castrate conditions in long term culture in order to enrich for the population of cells harboring a 48kb intragenic deletion in AR intron 1. These late-passage CWR-R1 cells were electroporated with siRNAs targeting AR exon 1, AR exon 7, or AR exon CE3. Electroporated cells were seeded in RPMI 1640 medium containing antibiotics and 5% charcoal-stripped, steroid-depleted medium and allowed to recover for 48h. After this 48h recovery, electroporated cells were switched to serum-free RPMI 1640 with 1nM DHT or 0.1% ethanol (vehicle control) for an additional 24h prior to extraction of total RNA. Three independent biological replicates were performed.
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| Contributor(s) |
Dehm SM |
| Citation(s) |
23117885 |
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| Submission date |
Oct 23, 2012 |
| Last update date |
Jan 28, 2016 |
| Contact name |
Scott Dehm |
| E-mail(s) |
dehm@umn.edu
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| Organization name |
Masonic Cancer Center, University of Minnesota
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| Street address |
Mayo Mail Code 806, 420 Delaware St SE
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| City |
Minneapolis |
| ZIP/Postal code |
55455 |
| Country |
USA |
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| Platforms (1) |
| GPL10904 |
Illumina HumanHT-12 V4.0 expression beadchip (gene symbol) |
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| Samples (12)
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| GSM1024279 |
siRNA targeted to AR exon 7 treated with ethanol, replicate 1 |
| GSM1024280 |
siRNA targeted to AR exon CE3 treated with ethanol, replicate 2 |
| GSM1024281 |
siRNA targeted to AR exon 1 treated with ethanol, replicate 1 |
| GSM1024282 |
siRNA targeted to AR exon CE3 treated with 1nM DHT, replicate 2 |
| GSM1024283 |
siRNA targeted to AR exon CE3 treated with ethanol, replicate 1 |
| GSM1024284 |
siRNA targeted to AR exon 7 treated with ethanol, replicate 3 |
| GSM1024285 |
siRNA targeted to AR exon CE3 treated with 1nM DHT, replicate 1 |
| GSM1024286 |
siRNA targeted to AR exon 1 treated with ethanol, replicate 3 |
| GSM1024287 |
siRNA targeted to AR exon 7 treated with ethanol, replicate 2 |
| GSM1024288 |
siRNA targeted to AR exon CE3 treated with ethanol, replicate 3 |
| GSM1024289 |
siRNA targeted to AR exon 1 treated with ethanol, replicate 2 |
| GSM1024290 |
siRNA targeted to AR exon CE3 treated with 1nM DHT, replicate 3 |
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| Relations |
| BioProject |
PRJNA178169 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE41784_non-normalized_data.txt.gz |
3.6 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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