|
| Status |
Public on Oct 23, 2012 |
| Title |
siRNA targeted to AR exon CE3 treated with 1nM DHT, replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
late-passage CWR-R1 prostate cancer cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: CWR-R1 prostate cancer cells
genotype/variation: 48kb intragenic deletion in AR intron 1
sirna: AR exon CE3
biological replicate number: rep3
agent: DHT
|
| Treatment protocol |
48h post-transfection with the appropriate siRNA, cells were stimulated with 1nM DHT or vehicle control (ethanol) for 24h prior to RNA extraction.
|
| Growth protocol |
Late-passage CWR-R1 cells were cultured in RPMI 1640 medium supplemented with penicillin/streptomycin and 5% charcoal-stripped, steroid-depleted serum for 48h following electroporation. Cells were then switched to serum-free RPMI 1640 medium supplemented with penicillin/streptomycin containing 1nM DHT or vehicle control (ethanol) for 24h.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using an acid guanidinium method and cleaned using a QIAGEN RNeasy kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with an a NanoDrop 8000 spectrophotometer and Caliper LabChip GX.
|
| Label |
biotin
|
| Label protocol |
Total RNA was converted to amplified, biotinylated, antisense cRNA using the Illumina TotalPrep-96 RNA Amplification Kit (Life Technologies) in accordance with the manufacturer's specifications.
|
| |
|
| Hybridization protocol |
150ng of biotin-labeled cRNA was hybridized with Illumina Beadchips using the HumanHT-12 v4 Expression Beadchip Kit in accordance with the manufacturer's specifications.
|
| Scan protocol |
Hybridized Beadchips were scanned with an Illumina iScan.
|
| Description |
SAMPLE 12
|
| Data processing |
Average probe intensity data was extracted from iScan image files using the Partek Report Plug-in for Illumina GenomeStudio and then imported to Partek Genomics Suite 6.6. Raw intensity data was log2 transformed and quantile normalized using Partek GS 6.6.
|
| |
|
| Submission date |
Oct 23, 2012 |
| Last update date |
Oct 23, 2012 |
| Contact name |
Scott Dehm |
| E-mail(s) |
dehm@umn.edu
|
| Organization name |
Masonic Cancer Center, University of Minnesota
|
| Street address |
Mayo Mail Code 806, 420 Delaware St SE
|
| City |
Minneapolis |
| ZIP/Postal code |
55455 |
| Country |
USA |
| |
|
| Platform ID |
GPL10904 |
| Series (1) |
| GSE41784 |
Androgen receptor splice variants mediate enzalutamide resistance in castration-resistant prostate cancer cell lines |
|
