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| Status |
Public on May 01, 2013 |
| Title |
Gene Expression in Forebrain of Male Goldfish Following Exposure to Waterborne Sex Pheromones |
| Platform organisms |
Carassius auratus; Cyprinus carpio |
| Sample organism |
Carassius auratus |
| Experiment type |
Expression profiling by array
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| Summary |
Sex pheromones rapidly affect endocrine physiology and behaviour, but little is known about their effects on gene expression in the neuroendocrine tissues that mediate olfactory processing. In this study we exposed male goldfish for 6 h to waterborne 17,20betaP (4.3 nM) and PGF2α (3 nM), the main pre-ovulatory and post-ovulatory pheromones, respectively. Both treatments elevated milt volume (P = 0.001). Microarray hybridizations were used to male telencephalon samples following PGF2α treatment and identified 71 unique transcripts that were differentially expressed (q < 5%; 67 up, 4 down). Functional annotation of these regulated genes indicates that PGF2α pheromone exposure affects diverse biological processes including nervous system functions, energy metabolism, cholesterol/lipoprotein transport, translational regulation, transcription and chromatin remodelling, protein processing, cytoskeletal organization, and signalling. By using real-time RT-PCR, we further validated three candidate genes, ependymin-II, calmodulin-A and aldolase C, which exhibited 3 - 5 fold increase in expression following PGF2α exposure. Expression levels of some other genes that are thought to be important for reproduction were also determined using real-time RT-PCR. Expression of sGnRH was increased by PGF2α, but not 17,20betaP, whereas cGnRH expression was increased by 17,20betaP but not PGF2α. In contrast, both pheromones increase the expression of glutamate (GluR2a, NR2A) and gamma-aminobutyric acid (GABAA gamma2) receptor subunit mRNAs. This gene expression data link milt release and rapid modulation of neuronal transcription as part of the response to female sex pheromones.
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| Overall design |
A total of four microarray slides were hybridized to study the effects of PGF2α on gene expression in the telencephalon. Control samples (N = 4) were pooled to form our reference or technical replicates while treatment samples (N = 4), which formed our biological replicates, were ran separately. In addition, two dye swaps were performed for our biological samples for the treatments.
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| Contributor(s) |
Lado WE, Zhang D, Mennigen JA, Zamora JM, Popesku JT, Trudeau VL |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Oct 30, 2012 |
| Last update date |
May 01, 2013 |
| Contact name |
Wudu E. Lado |
| E-mail(s) |
wlado038@uottawa.ca
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| Phone |
613-562-6015
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| Fax |
613-562-5486
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| Organization name |
University of Ottawa
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| Department |
Biology
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| Lab |
Trudeau
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| Street address |
30 Marie Private
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| City |
Ottawa |
| State/province |
ON |
| ZIP/Postal code |
K1N 6N5 |
| Country |
Canada |
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| Platforms (1) |
| GPL7056 |
AURATUS: The goldfish environmental genomics project 17K array v1.1 |
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| Samples (4)
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| GSM1027374 |
Gene Expression in Forebrain of Male Goldfish Following Exposure to Waterborne Sex Pheromones Replicate 1 |
| GSM1027375 |
Gene Expression in Forebrain of Male Goldfish Following Exposure to Waterborne Sex Pheromones Replicate 2 |
| GSM1027376 |
Gene Expression in Forebrain of Male Goldfish Following Exposure to Waterborne Sex Pheromones Replicate 3 |
| GSM1027377 |
Gene Expression in Forebrain of Male Goldfish Following Exposure to Waterborne Sex Pheromones Replicate 4 |
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| Relations |
| BioProject |
PRJNA179219 |
| Supplementary data files not provided |
| Processed data included within Sample table |
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