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| Status |
Public on Nov 02, 2012 |
| Title |
In vivo NCL-targeting affects breast cancer aggressiveness through miRNA regulation [Affymetrix] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Numerous studies have described the altered expression and the causal role of miRNAs in human cancer. However, to date efforts to modulate miRNA levels for therapeutic purposes have been challenging to implement. Here, we find that Nucleolin (NCL), a major nucleolar protein, post-transcriptionally regulates the expression of a specific subset of miRNAs, including miR-21, miR-221, miR-222, and miR-103, causally involved in breast cancer initiation, progression and drug-resistance. We also show that NCL is commonly overexpressed in human breast tumors, and its expression correlates with that of NCL-dependent miRNAs. Finally, this study indicates that NCL-binding guanosine-rich aptamers affect the levels of NCL-dependent miRNAs and their target genes, reducing breast cancer cell aggressiveness, both in vitro and in vivo. These findings illuminate a path to novel therapeutic approaches based on NCL-targeting aptamers for the modulation of miRNA expression in the treatment of breast cancer.
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| Overall design |
MCF7 cells were treated with the control drug or AS1411 aptamer. After 72hours total RNA was collected and analyzed by Affymetrix U133 plus.
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| Contributor(s) |
Pichiorri F, Palmieri D, De Luca L, Consiglio J, You J, Rocci A, Talabere T, Pivan C, Lagana A, Cascione L, Guan J, Gasparini P, Balatti V, Coppola V, Hofmeister CC, Marcucci G, Byrd JC, Volinia S, Shapiro CL, Freitas MA, Croce CM |
| Citation(s) |
23610125 |
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| Submission date |
Nov 01, 2012 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Luciano Cascione |
| E-mail(s) |
luciano.cascione@ior.usi.ch
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| Organization name |
IOR
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| Department |
Lymphoma & Genomics Research Program
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| Street address |
Via Chiesa 5
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| City |
Bellinzona |
| State/province |
Ticino |
| ZIP/Postal code |
6500 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE41973 |
In vivo NCL-targeting affects breast cancer aggressiveness through miRNA regulation |
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| Relations |
| BioProject |
PRJNA178628 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE41972_RAW.tar |
30.1 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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