|
| Status |
Public on Nov 02, 2012 |
| Title |
as1411 MCF7-72h-2 |
| Sample type |
RNA |
| |
|
| Source name |
MCF7 cells treated with AS1411 aptamer
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF7
|
| Treatment protocol |
MCF7 cells were treated with the control drug or AS1411 aptamer. After 72hours total RNA was collected and analyzed by Affymetrix U133 plus.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
See Affymetrix protocol.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from MCF7 cells treated with AS1411 aptamer
|
| Data processing |
The data were analyzed with RMA.
|
| |
|
| Submission date |
Nov 01, 2012 |
| Last update date |
Nov 02, 2012 |
| Contact name |
Luciano Cascione |
| E-mail(s) |
luciano.cascione@ior.usi.ch
|
| Organization name |
IOR
|
| Department |
Lymphoma & Genomics Research Program
|
| Street address |
Via Chiesa 5
|
| City |
Bellinzona |
| State/province |
Ticino |
| ZIP/Postal code |
6500 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE41972 |
In vivo NCL-targeting affects breast cancer aggressiveness through miRNA regulation [Affymetrix] |
| GSE41973 |
In vivo NCL-targeting affects breast cancer aggressiveness through miRNA regulation |
|
