|
Status |
Public on Nov 02, 2012 |
Title |
as1411 MCF7-72h-2 |
Sample type |
RNA |
|
|
Source name |
MCF7 cells treated with AS1411 aptamer
|
Organism |
Homo sapiens |
Characteristics |
cell line: MCF7
|
Treatment protocol |
MCF7 cells were treated with the control drug or AS1411 aptamer. After 72hours total RNA was collected and analyzed by Affymetrix U133 plus.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
|
|
Hybridization protocol |
See Affymetrix protocol.
|
Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
Description |
Gene expression data from MCF7 cells treated with AS1411 aptamer
|
Data processing |
The data were analyzed with RMA.
|
|
|
Submission date |
Nov 01, 2012 |
Last update date |
Nov 02, 2012 |
Contact name |
Luciano Cascione |
E-mail(s) |
luciano.cascione@ior.usi.ch
|
Organization name |
IOR
|
Department |
Lymphoma & Genomics Research Program
|
Street address |
Via Chiesa 5
|
City |
Bellinzona |
State/province |
Ticino |
ZIP/Postal code |
6500 |
Country |
Switzerland |
|
|
Platform ID |
GPL570 |
Series (2) |
GSE41972 |
In vivo NCL-targeting affects breast cancer aggressiveness through miRNA regulation [Affymetrix] |
GSE41973 |
In vivo NCL-targeting affects breast cancer aggressiveness through miRNA regulation |
|