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| Status |
Public on Nov 06, 2012 |
| Title |
Characterization of gene expression profiles induced by HIC-1 reactivation on breast cancer |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To explore the molecular mechanisms and signal pathways induced by restoring tumor suppressor gene HIC-1 on breast cancer cells. We have employed whole genome microarray expression profiling as a discovery platform to identify the differential genes induced by HIC-1 gene activation. Small activating RNA (saRNA) that targeted promoter region was used, and MCF-7 breast cancer cell line was selected as cell model. After 96h for saRNA transfection, the cells were collected and the whole genome expression profiles were analyzed. Three independent experiments were repeated for different groups. With the treshold of p<0.01 and fold change >=2 or <-2, there were 1375 differential expression genes, which are related to cell cycle, apoptosis, cell migration, cell invasion and cell proliferation.
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| Overall design |
SaRNA induced gene expression in human breast cancer cell MCF-7 was measured at 96 hours after transfection by 50 nM saRNA. Three independent experiments were performed for experimental group and control group.
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| Contributor(s) |
Yu Y, Zhang W |
| Citation(s) |
24489730 |
| |
| Submission date |
Nov 05, 2012 |
| Last update date |
Feb 04, 2020 |
| Contact name |
Yingyan Yu |
| E-mail(s) |
yingyan3y@yahoo.com.cn
|
| Phone |
86-21-64370045
|
| Fax |
86-21-64373909
|
| Organization name |
Shanghai Ruijin Hospital
|
| Department |
Department of Surgery
|
| Street address |
Shanghai Ruijin 2nd Road No.197
|
| City |
Shanghai |
| ZIP/Postal code |
200025 |
| Country |
China |
| |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
|
| Samples (6)
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| Relations |
| BioProject |
PRJNA178940 |
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