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Series GSE4223 Query DataSets for GSE4223
Status Public on Nov 18, 2008
Title Studying Gene Expression Profiles of UV-Irradiated Human Keratinocytes by cDNA Microarray
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The goal of this thesis is to study the response of a human keratinocyte cell line (HaCaT cells) after UVB irradiation. In order to develop a system for UV irradiation, we firtstly studied the UV illumination system to find out a stable and reliable condition for UV irradiation experiments. Further, we found out the proper dose of UVB radiation and time points after UVB irradiation by performing MTT assay and trypan blue viability test. According to the results of MTT assay and trypan blue viability test, the cellular activity as well as survival rate of UVB-irradiated cells were in proportion to the radiation doses within a lower UVB dose range. We wanted to find out the possible reasons for this phenomenon by using cDNA microarray. We hope the thesis could be a guide for the following researches, and be useful in the clinical studies about the UV damage.
Keywords: dose response and time course
 
Overall design Loop-designed microarray experiments were performed in our study. Twenty-one samples are arranges as seven small loops and one large loop, with three and seven microarray slides for each small and large loop, respectively. This set of loop-designed microarray experiment contains one control untreated (non UVB-treated) samples and two UVB-treated samples (one low dose and one high dose UVB-treated) at each time point (total seven time points).
 
Contributor(s) Chang C, Huang C
Citation(s) 30083841
Submission date Feb 09, 2006
Last update date Aug 16, 2018
Contact name ChengWei Chang
E-mail(s) d924546@oz.nthu.edu.tw
Organization name nthu
Department bmes
Lab mbpl
Street address 101, Section 2 Kuang Fu Road
City Hsinchu
ZIP/Postal code 30013
Country Taiwan
 
Platforms (1)
GPL2573 MBPL human 30k P2
Samples (28)
GSM96319 HaCaT_4hr_Ctrl/ low dose UVB (Y10_C4L4)
GSM96320 HaCaT_4hr_low dose/ high dose UVB (Y11_L4H4)
GSM96321 HaCaT_4hr_high dose/ Ctrl (Y12_H4C4)
Relations
BioProject PRJNA94867

Loop design adjacency matrix (1: Cy5, -1: Cy3) header descriptions
no
GEO_ACC
C1
L1 Sample harvested at 0.5 hr after low dose UVB irradiation (233 J/m2)
H1 Sample harvested at 0.5 hr after low dose UVB irradiation (582.5 J/m2)
C2 Control samples at 1 hr (no UVB-treated, corresponding to L2 and H2)
L2 Sample harvested at 1 hr after low dose UVB irradiation (233 J/m2)
H2 Sample harvested at 1 hr after low dose UVB irradiation (582.5 J/m2)
C3 Control samples at 2 hr (no UVB-treated, corresponding to L3 and H3)
L3 Sample harvested at 2 hr after low dose UVB irradiation (233 J/m2)
H3 Sample harvested at 2 hr after low dose UVB irradiation (582.5 J/m2)
C4 Control samples at 4 hr (no UVB-treated, corresponding to L4 and H4)
L4 Sample harvested at 4 hr after low dose UVB irradiation (233 J/m2)
H4 Sample harvested at 4 hr after low dose UVB irradiation (582.5 J/m2)
C5 Control samples at 8 hr (no UVB-treated, corresponding to L5 and H5)
L5 Sample harvested at 8 hr after low dose UVB irradiation (233 J/m2)
H5 Sample harvested at 8 hr after low dose UVB irradiation (582.5 J/m2)
C6 Control samples at 16 hr (no UVB-treated, corresponding to L6 and H6)
L6 Sample harvested at 16 hr after low dose UVB irradiation (233 J/m2)
H6 Sample harvested at 16 hr after low dose UVB irradiation (582.5 J/m2)
C7 Control samples at 20 hr (no UVB-treated, corresponding to L7 and H7)
L7 Sample harvested at 20 hr after low dose UVB irradiation (233 J/m2)
H7 Sample harvested at 20 hr after low dose UVB irradiation (582.5 J/m2)
Title

Data table
no GEO_ACC C1 L1 H1 C2 L2 H2 C3 L3 H3 C4 L4 H4 C5 L5 H5 C6 L6 H6 C7 L7 H7 Title
1 GSM96329 -1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Y1_C1L1
2 GSM96339 0 -1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Y2_L1H1
3 GSM96340 1 0 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Y3_H1C1
4 GSM96341 0 0 0 -1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Y4_C2L2
5 GSM96342 0 0 0 0 -1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Y5_L2H2
6 GSM96343 0 0 0 1 0 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Y6_H2C2
7 GSM96344 0 0 0 0 0 0 -1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 Y7_C3L3
8 GSM96345 0 0 0 0 0 0 0 -1 1 0 0 0 0 0 0 0 0 0 0 0 0 Y8_L3H3
9 GSM96346 0 0 0 0 0 0 1 0 -1 0 0 0 0 0 0 0 0 0 0 0 0 Y9_H3C3
10 GSM96319 0 0 0 0 0 0 0 0 0 -1 1 0 0 0 0 0 0 0 0 0 0 Y10_C4L4
11 GSM96320 0 0 0 0 0 0 0 0 0 0 -1 1 0 0 0 0 0 0 0 0 0 Y11_L4H4
12 GSM96321 0 0 0 0 0 0 0 0 0 1 0 -1 0 0 0 0 0 0 0 0 0 Y12_H4C4
13 GSM96322 0 0 0 0 0 0 0 0 0 0 0 0 -1 1 0 0 0 0 0 0 0 Y13_C5L5
14 GSM96323 0 0 0 0 0 0 0 0 0 0 0 0 0 -1 1 0 0 0 0 0 0 Y14_L5H5
15 GSM96324 0 0 0 0 0 0 0 0 0 0 0 0 1 0 -1 0 0 0 0 0 0 Y15_H5C5
16 GSM96325 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 -1 1 0 0 0 0 Y16_C6L6
17 GSM96326 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 -1 1 0 0 0 Y17_L6H6
18 GSM96327 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 -1 0 0 0 Y18_H6C6
19 GSM96328 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 -1 1 0 Y19_C7L7
20 GSM96330 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 -1 1 Y20_L7H7

Total number of rows: 28

Table truncated, full table size 1 Kbytes.




Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE4223.pdf 28.0 Kb (ftp)(http) PDF
GSE4223_RAW.tar 60.1 Mb (http)(custom) TAR (of GPR)

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