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Status |
Public on Nov 18, 2008 |
Title |
Studying Gene Expression Profiles of UV-Irradiated Human Keratinocytes by cDNA Microarray |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
The goal of this thesis is to study the response of a human keratinocyte cell line (HaCaT cells) after UVB irradiation. In order to develop a system for UV irradiation, we firtstly studied the UV illumination system to find out a stable and reliable condition for UV irradiation experiments. Further, we found out the proper dose of UVB radiation and time points after UVB irradiation by performing MTT assay and trypan blue viability test. According to the results of MTT assay and trypan blue viability test, the cellular activity as well as survival rate of UVB-irradiated cells were in proportion to the radiation doses within a lower UVB dose range. We wanted to find out the possible reasons for this phenomenon by using cDNA microarray. We hope the thesis could be a guide for the following researches, and be useful in the clinical studies about the UV damage. Keywords: dose response and time course
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Overall design |
Loop-designed microarray experiments were performed in our study. Twenty-one samples are arranges as seven small loops and one large loop, with three and seven microarray slides for each small and large loop, respectively. This set of loop-designed microarray experiment contains one control untreated (non UVB-treated) samples and two UVB-treated samples (one low dose and one high dose UVB-treated) at each time point (total seven time points).
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Contributor(s) |
Chang C, Huang C |
Citation(s) |
30083841 |
Submission date |
Feb 09, 2006 |
Last update date |
Aug 16, 2018 |
Contact name |
ChengWei Chang |
E-mail(s) |
d924546@oz.nthu.edu.tw
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Organization name |
nthu
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Department |
bmes
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Lab |
mbpl
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Street address |
101, Section 2 Kuang Fu Road
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City |
Hsinchu |
ZIP/Postal code |
30013 |
Country |
Taiwan |
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Platforms (1) |
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Samples (28)
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GSM96319 |
HaCaT_4hr_Ctrl/ low dose UVB (Y10_C4L4) |
GSM96320 |
HaCaT_4hr_low dose/ high dose UVB (Y11_L4H4) |
GSM96321 |
HaCaT_4hr_high dose/ Ctrl (Y12_H4C4) |
GSM96322 |
HaCaT_8hr_Ctrl/ low dose UVB (Y13_C5L5) |
GSM96323 |
HaCaT_8hr_low dose/ high dose UVB (Y14_L5H5) |
GSM96324 |
HaCaT_8hr_high dose UVB/ Ctrl (Y15_H5C5) |
GSM96325 |
HaCaT_16hr_Ctrl/ low dose UVB (Y16_C6L6) |
GSM96326 |
HaCaT_16hr_low dose/ high dose UVB (Y17_L6H6) |
GSM96327 |
HaCaT_16hr_high dose UVB/ Ctrl (Y18_H6C6) |
GSM96328 |
HaCaT_20hr_Ctrl/ low dose UVB (Y19_C7L7) |
GSM96329 |
HaCaT_0.5hr_Ctrl/ low dose UVB (Y1_C1L1) |
GSM96330 |
HaCaT_20hr_low dose/ high dose UVB (Y20_L7H7) |
GSM96331 |
HaCaT_20hr_high dose UVB/ Ctrl (Y21_H7C7) |
GSM96332 |
HaCaT_0.5hr vs 1hr_low dose UVB (Y22_L1L2) |
GSM96333 |
HaCaT_1hr vs 2hr_low dose UVB (Y23_L2L3) |
GSM96334 |
HaCaT_2hr vs 4hr_low dose UVB (Y24_L3L4) |
GSM96335 |
HaCaT_4hr vs 8hr_low dose UVB (Y25_L4L5) |
GSM96336 |
HaCaT_8hr vs 16hr_low dose UVB (Y26_L5L6) |
GSM96337 |
HaCaT_16hr vs 20hr_low dose UVB (Y27_L6L7) |
GSM96338 |
HaCaT_20hr vs 0.5hr_low dose UVB (Y28_L7L1) |
GSM96339 |
HaCaT_0.5hr_low dose/ high dose UVB (Y2_L1H1) |
GSM96340 |
HaCaT_0.5hr_high dose UVB/ Ctrl ( Y3_H1C1) |
GSM96341 |
HaCaT_1hr_Ctrl/ low dose UVB (Y4_C2L2) |
GSM96342 |
HaCaT_1hr_ low dose/ high dose UVB (Y5_L2H2) |
GSM96343 |
HaCaT_1hr_ high dose UVB/ Ctrl (Y6_H2C2) |
GSM96344 |
HaCaT_2hr_ Ctrl/ low dose UVB (Y7_C3L3) |
GSM96345 |
HaCaT_2hr_low dose/ high dose UVB (Y8_L3H3) |
GSM96346 |
HaCaT_2hr_high dose UVB/ Ctrl (Y9_H3C3) |
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Relations |
BioProject |
PRJNA94867 |
Loop design adjacency matrix (1: Cy5, -1: Cy3) header descriptions |
no |
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GEO_ACC |
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C1 |
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L1 |
Sample harvested at 0.5 hr after low dose UVB irradiation (233 J/m2) |
H1 |
Sample harvested at 0.5 hr after low dose UVB irradiation (582.5 J/m2) |
C2 |
Control samples at 1 hr (no UVB-treated, corresponding to L2 and H2) |
L2 |
Sample harvested at 1 hr after low dose UVB irradiation (233 J/m2) |
H2 |
Sample harvested at 1 hr after low dose UVB irradiation (582.5 J/m2) |
C3 |
Control samples at 2 hr (no UVB-treated, corresponding to L3 and H3) |
L3 |
Sample harvested at 2 hr after low dose UVB irradiation (233 J/m2) |
H3 |
Sample harvested at 2 hr after low dose UVB irradiation (582.5 J/m2) |
C4 |
Control samples at 4 hr (no UVB-treated, corresponding to L4 and H4) |
L4 |
Sample harvested at 4 hr after low dose UVB irradiation (233 J/m2) |
H4 |
Sample harvested at 4 hr after low dose UVB irradiation (582.5 J/m2) |
C5 |
Control samples at 8 hr (no UVB-treated, corresponding to L5 and H5) |
L5 |
Sample harvested at 8 hr after low dose UVB irradiation (233 J/m2) |
H5 |
Sample harvested at 8 hr after low dose UVB irradiation (582.5 J/m2) |
C6 |
Control samples at 16 hr (no UVB-treated, corresponding to L6 and H6) |
L6 |
Sample harvested at 16 hr after low dose UVB irradiation (233 J/m2) |
H6 |
Sample harvested at 16 hr after low dose UVB irradiation (582.5 J/m2) |
C7 |
Control samples at 20 hr (no UVB-treated, corresponding to L7 and H7) |
L7 |
Sample harvested at 20 hr after low dose UVB irradiation (233 J/m2) |
H7 |
Sample harvested at 20 hr after low dose UVB irradiation (582.5 J/m2) |
Title |
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