kindly provided by Dr. P. Boukamp, DKFZ, Heidelberg, Germany through Dr. T.C., Lee, SINICA, Taipei, Taiwan
Treatment protocol
Cells exposed to UVB 233 J/m2 , and then incubate for 4 hr
Growth protocol
Cells were maintained at 37 oC and 5 % CO2 /95% air in DMEM (Gibico) supplemented with 2mM L-glutamine, 100 U/ml penicillin/ 100£gg/ml streptomycin(Gibico), and 10% fetal bovine serum(Hyclone).
Extracted molecule
total RNA
Extraction protocol
Total RNA was extracte by lying directly in the 100mm petri dish with 2 ml TRIzol reagent (Invitrogen, Carlsbad, CA) following the manufacturer¡¦s instruction and then purified by RNeasy kit (Qiagen, Inc., Valencia, CA).
kindly provided by Dr. P. Boukamp, DKFZ, Heidelberg, Germany through Dr. T.C., Lee, SINICA, Taipei, Taiwan
Treatment protocol
Cells exposed to UVB 233 J/m2 , and then incubate for 2 hr
Growth protocol
Cells were maintained at 37 oC and 5 % CO2 /95% air in DMEM (Gibico) supplemented with 2mM L-glutamine, 100 U/ml penicillin/ 100£gg/ml streptomycin(Gibico), and 10% fetal bovine serum(Hyclone).
Extracted molecule
total RNA
Extraction protocol
Total RNA was extracte by lying directly in the 100mm petri dish with 2 ml TRIzol reagent (Invitrogen, Carlsbad, CA) following the manufacturer¡¦s instruction and then purified by RNeasy kit (Qiagen, Inc., Valencia, CA).
