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Series GSE42333 Query DataSets for GSE42333
Status Public on Dec 31, 2014
Title Effect of cigarette smoke extract, cisplatin, nicotine and/or ionizing radiation on microRNA expression in the NCI-H460 human lung large cell carcinoma cell line
Platform organisms Human alphaherpesvirus 1; Human alphaherpesvirus 2; Cytomegalovirus; Human betaherpesvirus 5; Lymphocryptovirus; Rhadinovirus; JC polyomavirus; Human immunodeficiency virus 1; Murid gammaherpesvirus 4; Merkel cell polyomavirus; Betapolyomavirus hominis; Betapolyomavirus macacae
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary To understand the effect of nicotine on sensitivity of cancer cells to radiation or anti-cancer drugs, NCI-H460 human lung large cell carcinoma cells were treated with 6 Gy ionizing radiation or 1 uM cisplatin after exposure to 0.5 uM nicotine or cigarette smoke extract. MicroRNA expression in total RNA extracted from the treated cells was quantified using the 7th generation miRCURY™ locked nucleic acid microarray platform from Exiqon® (Vedbaek, Denmark).
 
Overall design Cells were subjected to one of nine types of treatment in triplicate and harvested for RNA extraction after 2 or 24 hours. Thus, 54 RNA samples representing biological triplicates for nine types of treatments and two time-points were obtained and analyzed using microarrays. Three RNA samples were assayed in duplicate (thus, 3 sets of technical duplicates: samples 5 & 55, 7 & 56, and 28 & 57). Reference RNA created by pooling the 54 unique RNA samples (at equal concentrations) was used in the reference channel (channel 2) of all microarrays. The reference RNA was also assessed in the test channel (channel 1) of one microarray (i.e., both test and reference channels had the pooled RNA).
 
Contributor(s) Patnaik SK, Romano M, Sobus S, Pundt K, Yendamuri S, Warren G
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Submission date Nov 15, 2012
Last update date Dec 31, 2014
Contact name Santosh Kumar Patnaik
Phone 716-8458364
Organization name Roswell Park Comprehensive Cancer Center
Department Thoracic Surgery
Street address Elm and Carlton Streets
City Buffalo
State/province NY
ZIP/Postal code 14263
Country USA
 
Platforms (1)
GPL16287 Exiqon miRCURY LNA microRNA array; 7th generation; batch 208500-2, 208510; lot 35006 - hsa, mmu & rno (miRBase 19.0)
Samples (58)
GSM1038008 Control_2h_rep1
GSM1038009 Control_2h_rep2
GSM1038010 Control_2h_rep3
This SubSeries is part of SuperSeries:
GSE42334 Effect of cigarette smoke extract, cisplatin, nicotine and/or ionizing radiation on mRNA and microRNA expression in the NCI-H460 human lung large cell carcinoma cell line
Relations
BioProject PRJNA181093

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE42333_RAW.tar 159.3 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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