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| Status |
Public on Sep 01, 2013 |
| Title |
MicroRNA expression in prostate cancer cells stimulated with cancer associated fibroblasts |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Tumor microenvironment is a strong determinant for the acquisition of metastatic potential of cancer cells. It has been demonstrated (Giannoni E et al., Cancer Res 2010) that cancer associated fibroblasts (CAF) elicit an epithelial-mesenchymal transition (EMT) in prostate cancer (PCa) cells, leading to enhanced tumor cell aggressiveness. Here, we investigated the involvement of microRNAs (miRNA) in such malignant tumor-stroma interplay, to identify possible tools to counteract metastasis dissemination.
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| Overall design |
To verify whether specific miRNAs are involved in CAF-induced EMT in PCa cells, PC-3 cells were subjected to a variety of stimuli, known to induce or not the acquisition of a mesenchymal phenotype (Giannoni E et al., Cancer Res 2010; Giannoni E et al., Antioxid Redox Signal 2011) , and profiled for miRNA expression on a microarray platform. miRNA expression profiles successfully distinguished cells that underwent EMT following the stimulation with activated fibroblasts (here referred to as “mesenchymal”) from cells that did not (“epithelial”).
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| Contributor(s) |
Gandellini P |
| Citation(s) |
23924028 |
| |
| Submission date |
Dec 03, 2012 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Paolo Gandellini |
| E-mail(s) |
paolo.gandellini@istitutotumori.mi.it
|
| Organization name |
Fondazione IRCCS Istituto Nazionale Tumori
|
| Department |
Department of Applied Research and Technological Development
|
| Street address |
Via Amadeo 42
|
| City |
Milan |
| ZIP/Postal code |
20133 |
| Country |
Italy |
| |
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| Platforms (1) |
| GPL8179 |
Illumina Human v2 MicroRNA expression beadchip |
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| Samples (9)
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| GSM1048124 |
Unstimulated PC-3 cells |
| GSM1048125 |
PC-3 cells stimulated with the conditioned medium from prostatic fibroblasts |
| GSM1048126 |
PC-3 cells stimulated with the conditioned medium from prostatic fibroblasts, previously activated with TGF-β |
| GSM1048127 |
PC-3 cells stimulated with the conditioned medium from prostatic fibroblasts, previously activated with IL-6 |
| GSM1048128 |
PC-3 cells stimulated with the conditioned medium from prostate cancer-associated fibroblasts |
| GSM1048129 |
PC-3 cells silenced for COX-2 |
| GSM1048130 |
PC-3 cells silenced for COX-2, then stimulated with the conditioned medium from prostate cancer-associated fibroblasts |
| GSM1048131 |
PC-3 cells silenced for HIF1-α |
| GSM1048132 |
PC-3 cells silenced for HIF1-α, then stimulated with the conditioned medium from prostate cancer-associated fibroblasts |
|
| Relations |
| BioProject |
PRJNA182757 |
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