|
| Status |
Public on Sep 01, 2013 |
| Title |
PC-3 cells silenced for COX-2 |
| Sample type |
RNA |
| |
|
| Source name |
PC-3 cells, derived from prostate adenocarcinoma metastasis
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: PC-3
treatment: silenced for COX-2
emt status: epithelial
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol (Life technology), according to the manufacturer's protocol.
|
| Label |
Cy3
|
| Label protocol |
Total RNA (0.4 μg) was polyadenylated and converted to cDNA by standard methods. After PCR amplification, cDNA was fluorescent labelled using Cy3
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
PC-3 cells silenced for COX-2
PC-3_siCOX-2
5252223019_F
|
| Data processing |
Using BeadStudio software v3 and BeadStudio expression analysis module v3.3.8, the summary intensity given by the average bead intensities among all beads belonging to a particular miRNA was calculated along with the detection p-value; data were quantile normalized and exported with neither background nor scale correction. Filtering was performed using BRB-ArrayTools Version: 4.2.0 - ?_2 (June 2011). We excluded signals with detection p-value>0.05, and miRNAs when a) less than 15 % of expression data had at least a 1.2-fold change in either direction from gene's median value or b) Percent of data missing or filtered out exceeded 30%.
|
| |
|
| Submission date |
Dec 03, 2012 |
| Last update date |
Sep 01, 2013 |
| Contact name |
Paolo Gandellini |
| E-mail(s) |
paolo.gandellini@istitutotumori.mi.it
|
| Organization name |
Fondazione IRCCS Istituto Nazionale Tumori
|
| Department |
Department of Applied Research and Technological Development
|
| Street address |
Via Amadeo 42
|
| City |
Milan |
| ZIP/Postal code |
20133 |
| Country |
Italy |
| |
|
| Platform ID |
GPL8179 |
| Series (1) |
| GSE42699 |
MicroRNA expression in prostate cancer cells stimulated with cancer associated fibroblasts |
|
