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| Status |
Public on Sep 03, 2013 |
| Title |
Intracranial self-stimulation regulates gene expression in rat hippocampus |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
Transcriptional profiling of rat hippocampus 4.5h post intracranial self-stimulation (ICSS) show that this treatment, which improves learning and memory processes in a variety of paradigms of implicit and explicit memory, regulates gene expression of genes related to cAMP-mediated signaling and regulation of synaptic plasticity, among others.
Rats were implanted with a monopolar stainless steel electrode aimed at the lateral hypothalamus in the right hemisphere. Hippocampal gene expression was analyzed 4.5h post treatment comparing rats subjected to acute self-stimulation (2500 stimulation trains; ICSS group) with sham-operated rats (with electrode placement but placed in the ICSS without stimulation during 40 min.; Sham group).
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| Overall design |
Two-condition experiment, ICSS and Sham. Every sample consisted of pooled hippocampi ipsilateral to the electrode from three rats. Slices between Bregma levels -2 and -4 were used for dissections of the hippocampi. Common reference design, comparing every condition versus a brain pooled tissue consisting of pooled hippocampal tissue ipsilateral to the electrode of sham-operated (n=3) and stimulated (n=3) rats. Biological replicates: 4 DBS and 4 sham. One replicate per array.
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| Contributor(s) |
Huguet G, Kádár E, Aldavert-Vera L, Segura-Torres P |
| Citation(s) |
23898803 |
| Submission date |
Dec 06, 2012 |
| Last update date |
Dec 21, 2016 |
| Contact name |
Gemma Huguet |
| E-mail(s) |
gemma.huguet@udg.edu
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| Organization name |
Universitat de Girona
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| Street address |
Campus de Montilivi
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| City |
Girona |
| ZIP/Postal code |
17071 |
| Country |
Spain |
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| Platforms (1) |
| GPL4135 |
Agilent-014879 Whole Rat Genome Microarray 4x44K G4131F (Feature Number version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA183164 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE42793_RAW.tar |
54.6 Mb |
(http)(custom) |
TAR (of GPR) |
| Processed data included within Sample table |
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