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| Status |
Public on Dec 19, 2012 |
| Title |
Novel dedifferentiated liposarcoma xenograft models reveal PTEN down regulation as a malignant signature and response to PI3K pathway inhibition |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Liposarcoma is a type of soft tissue sarcoma, exhibiting poor survival and a high recurrence rate. Treatment is generally limited to surgery and radiation, emphasizing the need to understand this disease. Because very few in vivo and in vitro models can reproducibly recapitulate the human disease, we generated several xenograft models from surgically resected human dedifferentiated liposarcoma. Our study demonstrates that all xenografts recapitulate morphologic and gene expression characteristics of the patient tumors after continuous in vivo passages. Importantly, xenograftability is directly correlated with disease specific survival of liposarcoma patients. When treated with the PI3K/AKT/mTOR pathway inhibitor rapamycin alone or in combination with the multi-kinase inhibitor sorafenib, all xenografts responded with increased lipid content and a more differentiated gene expression profile.
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| Overall design |
One-color arrays:
Vehicle vs combination (rapamycin & sorafenib) treatment for 2 separate dedifferentiated liposarcoma xenografts.
Two-color arrays:
Comparison of patient tumor, several passages of xenograft generated from that tumor, and cells cultured from xenografted tumors.
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| Contributor(s) |
Smith KB, Tran LM, Tam BM, Shurell EM, Li Y, Braas D, Tap WD, Christofk HR, Dry SM, Eilber FC, Wu H |
| Citation(s) |
23416162 |
| |
| Submission date |
Dec 18, 2012 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Linh My Tran |
| E-mail(s) |
linhtran@ucla.edu
|
| Organization name |
University of Los Angeles
|
| Department |
Medicine - Pulmonary & Critical Care
|
| Lab |
Dubinett Lab
|
| Street address |
Box 951690, 37-131 CHS
|
| City |
Los Angeles |
| State/province |
California |
| ZIP/Postal code |
90095-1690 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
| GPL13607 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version) |
|
| Samples (30)
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| Relations |
| BioProject |
PRJNA183994 |
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