|
| Status |
Public on Jun 18, 2013 |
| Title |
Binding of the C. elegans dosage compensation complex protein DPY-27 |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Dosage Compensation is required to correct for uneven gene dose between the sexes. We utilized global run-on sequencing (GRO-seq) to examine how Caenorhabditis elegans dosage compensation reduces transcription of X-linked genes. To facilitate these experiments, we required accurate 5’-ends of genes that have been missing due to a co-transcriptional trans-splicing event common in nematodes. We developed a modified GRO-seq protocol to identify TSSs that are supported by transcription, and determined that TSSs lie more than 1 kb upstream of the previously annotated TSS for nearly one-quarter of all genes. We then investigated the changes that occur in transcriptionally engaged RNA Polymerase when dosage compensation is disrupted, and find that dosage compensation controls recruitment of RNA Polymerase to X-linked genes.
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| |
|
| Overall design |
Two biological replicates of ChIP with DPY-27 and a random rabbit IgG
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| |
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| Contributor(s) |
Kruesi WS, Core LJ, Waters CT, Lis JT, Meyer BJ |
| Citation(s) |
23795297 |
| |
| Submission date |
Dec 20, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Barbara J. Meyer |
| E-mail(s) |
bjmeyer@berkeley.edu
|
| Phone |
510 643 5583
|
| Organization name |
HHMI/UCB
|
| Department |
MCB
|
| Lab |
Meyer
|
| Street address |
16 Barker Hall #3204
|
| City |
Berkeley |
| State/province |
CA |
| ZIP/Postal code |
94720 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL9269 |
Illumina Genome Analyzer II (Caenorhabditis elegans) |
|
| Samples (2) |
|
| This SubSeries is part of SuperSeries: |
| GSE43087 |
Condensin Controls Recruitment of RNA Polymerase II to Achieve X-Chromosome Dosage Compensation |
|
| Relations |
| BioProject |
PRJNA184364 |
| SRA |
SRP017637 |
