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Series GSE43883 Query DataSets for GSE43883
Status Public on Sep 01, 2013
Title affy_ath_2012_05-Arabidopsis Ubiquitin-Like Proteases characterization
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary affy_ath_2012_05 - affy_ath_2012_05 - The main objective is to determinate the influence of Ubiquitin-Like Proteases in plant transcriptional modulation. The experiment will also allow us to find what transcriptional cues are particularly affected in the mutants relatively to wild-type.-Seeds were stratified for 3 days at 4ºC in the dark. Afterwards, seeds were surface sterilized, sown onto MS media and grown at 23ºC, with 100 µE light condition, in long days (16h L/8h D). Each MS plate was dived in four, corresponding to a genotype (Col, AB, SIZ1 or SAB), and 10 seeds were sown per genotype. After 10 days, seedlings were collected and frozen in liquid nitrogen. Each replicate was made of ~40 seedlings coming from 4 different MS plates. Frozen plant material was grounded in an eppendorf tube using a mini pestle. RNA was extracted using RNeasy Plant Mini kit (QIAGEN cat.74904), following the manufacturer instructions (QIAGEN, Hilden, Germany). The RNA samples were DNase treated (Recombinant DNase I, Takara Biotechnology, Dalian, China) and subsequently cleaned by column using RNeasy Plant Mini kit (QIAGEN, Hilden, Germany).
Overall design 12 arrays - ATH1; gene knock out
Col [wild type]
AB [knock out / insertional mutagen /T-Dna]
SIZ1 [knock out / insertional mutagen /T-Dna]
SAB [knock out / insertional mutagen /T-Dna]
Web link
Contributor(s) Huguet S, Bejarano E, Castro P, Balzergue S, Martin-Magniette M
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Submission date Jan 30, 2013
Last update date Jun 12, 2017
Contact name stephanie huguet
Organization name inra
Street address 2 rue gaston cremieux
City evry
ZIP/Postal code 91000
Country France
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (12)
GSM1074047 COL_1 with array type ATH1 from Affymetrix
GSM1074048 SIZ_3 with array type ATH1 from Affymetrix
GSM1074049 COL_2 with array type ATH1 from Affymetrix
BioProject PRJNA188668

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE43883_RAW.tar 18.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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