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Status
Public on Sep 01, 2013
Title
COL_3 with array type ATH1 from Affymetrix
Sample type
RNA
Source name
COL_3
Organism
Arabidopsis thaliana
Characteristics
genotype/variation: COL
Treatment protocol
no treatment
Growth protocol
seedling - - Media: Murashige and Skoog (with Gamborg B5) medium - Temperature: 23ºC - Light: 100 µE
Extracted molecule
total RNA
Extraction protocol
COL_3:760ng.
Label
Biotin
Label protocol
labelling Biotin indirect, amplification=yes, ssDNA 5.5 ug.
Hybridization protocol
labelled extract quantity: 15ug
Scan protocol
GCOS, Biotin:pmt voltage 570nm,650V,laser power 1
Description
arabidopsis thaliana (columbia_0) - harvest date: 22-06-12 The main objective is to determinate the influence of Ubiquitin-Like Proteases in plant transcriptional modulation. The experiment will also allow us to find what transcriptional cues are particularly affected in the mutants relatively to wild-type.
Data processing
The data were normalized with the gcrma algorithm (Irizarry et al., 2003), available in the Bioconductor package (Gentleman and Carey, 2002).
Submission date
Jan 30, 2013
Last update date
Sep 01, 2013
Contact name
stephanie huguet
E-mail(s)
huguet@evry.inra.fr
Organization name
inra
Street address
2 rue gaston cremieux
City
evry
ZIP/Postal code
91000
Country
France
Platform ID
GPL198
Series (1)
GSE43883
affy_ath_2012_05-Arabidopsis Ubiquitin-Like Proteases characterization
Data table header descriptions
ID_REF
ID number
VALUE
Normalized data by GCRMA
Data table
ID_REF
VALUE
244901_at
2.46435370784677
244902_at
2.63728753509846
244903_at
3.18889888572981
244904_at
2.16739526913133
244905_at
2.25108664384513
244906_at
2.77028461100529
244907_at
2.47598895798693
244908_at
2.40985273650609
244909_at
2.32869374399437
244910_s_at
2.36656141696510
244911_at
2.61155202868983
244912_at
3.89430292509457
244913_at
2.18674291591398
244914_at
2.61443027927716
244915_s_at
2.29099584840996
244916_at
2.20450214101067
244917_at
2.28624895480641
244918_at
2.369815394083
244919_at
2.20124511793926
244920_s_at
3.2414064391663
Total number of rows: 22810 Table truncated, full table size 602 Kbytes .Supplementary file
Size
Download
File type/resource
GSM1074050_COL_3.CEL.gz
1.6 Mb
(ftp) (http)
CEL
Processed data included within Sample table