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| Status |
Public on Jul 11, 2006 |
| Title |
Expression data from primitive and maturing hematopoietic stem cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Gene expression studies from hematopoietic stem cell (HSC) populations purified to variable degrees have defined a set of stemness genes. The present study describes the construction and comparative molecular analysis of l-phage cDNA libraries from highly purified primitive HSCs (PHSCs) which retained their long term repopulating activities (LTRAs), and from maturing HSCs (MHSCs) which were largely depleted of LTRAs. Library inserts were amplified and tagged by a T7 RNA polymerase promoter and used to generate biotinylated cRNA for Microarray hybridization. Microarray analysis of the libraries confirmed previous results but also revealed an unforseen preferential expression of translation and metabolism associated genes in the PHSCs. Therefore these data indicate that HSCs are quiescent only in regard of proliferative activities, but are in a state of readiness to provide the metabolic and translational activities required following induction of proliferation by factors which induce differentiation and exit from the HSC pool.
We used microarrays to detail the global programme of gene expression distinguishing primitive and maturing hematopoietic stem cells from mouse bone marrow and identified distinct classes of up- and down-regulated genes.
Keywords: Adult hematopoietic stem cell populations
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| Overall design |
To compare the transcriptosomes of primitive and maturing hematopoietic stem cells from mouse bone marrow, cDNA libraries were generated from RNA isolated from highly purified stem cell populations and used to generate biotinylated cRNA for Affymetrix microarray analysis.
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| Contributor(s) |
Huettmann A, Duehrsen U, Heydarian K, Carpinteiro A, Klein-Hitpass L, Boes T, Boyd WA, Li CL |
| Citation(s) |
16574753 |
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| Submission date |
Mar 07, 2006 |
| Last update date |
Jan 08, 2019 |
| Contact name |
Ludger Klein-Hitpass |
| E-mail(s) |
ludger.klein-hitpass@uni-essen.de
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| Phone |
+49 201 723 85552
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| Organization name |
Institut fuer Zellbiologie
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| Department |
Universitaetsklinikum
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| Lab |
BioChip Lab
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| Street address |
Virchowstr. 173
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| City |
Essen |
| ZIP/Postal code |
D-45122 |
| Country |
Germany |
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| Platforms (1) |
| GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
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| Samples (2) |
| GSM99337 |
Maturing hematopoietic stem cells |
| GSM99338 |
Primitive hematopoietic stem cells |
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| Relations |
| BioProject |
PRJNA94579 |
