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| Status |
Public on Mar 01, 2013 |
| Title |
Genome scale analysis of host-virus mRNA differential expression in Helicoverpa zea insect cells infected by H. armigera baculovirus from early till late infection |
| Platform organisms |
Helicoverpa zea; Helicoverpa armigera |
| Sample organism |
Helicoverpa zea |
| Experiment type |
Expression profiling by array
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| Summary |
A custom microarray, based on deep transcriptome sequencing ((GEO accession number: GSE34418), was used to simultaneously investigate expression of over 24,000 Helicoverpa zea insect transcripts and 134 H. armigera nucleopolyhedrovirus (HearNPV) genes throughout the infection process at 0, 12, 24 and 48 hours post infection
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| Overall design |
A cutom 8x60,000 SurePrint Agilent expression slide (Agilent, Santa Clara, CA) was used to analyzed eight samples, including biological replicates of HaSNPV-infected cultures at 0, 12, 24 and 48 hpi.The microarray probes included probes for 27,400 H. zea sequences that were validated previously (Nguyen et al., 2012. PLOS ONE, 7(5), e36324) and all 134 H. armigera single-capsid nucleopolyhedrovirus (HearNPV) genes (Accession: NC_002654).
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| Contributor(s) |
Nguyen QH, Chan LC, Reid S, Nielsen LK |
| Citation(s) |
23827436 |
| |
| Submission date |
Feb 08, 2013 |
| Last update date |
Nov 15, 2014 |
| Contact name |
Quan Hoang Nguyen |
| E-mail(s) |
quan.nguyen@uq.edu.au
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| Phone |
(+61)733463147
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| Organization name |
The University of Queensland
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| Department |
Australian Institute for Bioengineering and Nanotechnology
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| Lab |
Single cell and computational genomics lab
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| Street address |
Queensland Bioscience Precinct (Building 80) The University of Queensland. 306 Carmody Road. St
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| City |
Brisbane |
| State/province |
Queensland |
| ZIP/Postal code |
4067 |
| Country |
Australia |
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| Platforms (1) |
| GPL16487 |
Agilent-037288 Timecourse_Insect_Virus |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA189039 |
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