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| Status |
Public on Feb 15, 2013 |
| Title |
Gene expression profile analysis of mouse whole spleen following infection by lymphocytic choriomeningitis virus (LCMV), comparing LCMV-Armstrong and LCMV-Clone 13 expression patterns at 0, 5, 9, and 30 days |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To identify mechanisms behind immunosuppression during virus infections, we infected mice with LCMV-Armstrong and LCMV-Clone 13 expression patterns. LCMV-Armstrong induces a T-cell reaction that resolves infection within 8-10 days, while LCMV-Clone13 generates a persisten infection through immunosuppression.
We used microarray to uncover splenic gene expression patterns specific to each LCMV infection at 5, 9, and 30 days
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| Overall design |
C57BL6 mice, 6-10 weeks old, were infected with LCMV-Armstrong and LCMV-Clone 13 or left uninfected (naïve). At days 5, 9, and 30 whole spleens were harvested for RNA extraction and hybridization on Affymetric microarray.
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| Contributor(s) |
Karns R, Aronow B, Brooks D |
| Citation(s) |
23580528 |
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| Submission date |
Feb 14, 2013 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Rebekah Karns |
| E-mail(s) |
Rebekah.Karns@cchmc.org
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| Organization name |
Cincinnati Children's Hospital Medical Center
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| Department |
Gastroenterology, Hepatology, & Nutrition
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| Street address |
3333 Burnet Ave
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| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45276 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (26)
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| GSM1083155 |
Uninfected spleen, day 0, rep4 |
| GSM1083156 |
LCMV-Armstrong spleen day 5, rep1 |
| GSM1083157 |
LCMV-Armstrong spleen day 5, rep2 |
| GSM1083158 |
LCMV-Armstrong spleen day 5, rep3 |
| GSM1083159 |
LCMV-Armstrong spleen day 9, rep1 |
| GSM1083160 |
LCMV-Armstrong spleen day 9, rep2 |
| GSM1083161 |
LCMV-Armstrong spleen day 9, rep3 |
| GSM1083162 |
LCMV-Armstrong spleen day 9, rep4 |
| GSM1083163 |
LCMV-Armstrong spleen day 30, rep1 |
| GSM1083164 |
LCMV-Armstrong spleen day 30, rep2 |
| GSM1083165 |
LCMV-Armstrong spleen day 30, rep3 |
| GSM1083166 |
LCMV-Armstrong spleen day 30, rep4 |
| GSM1083167 |
LCMV-Clone 13 spleen day 5, rep1 |
| GSM1083168 |
LCMV-Clone 13 spleen day 5, rep2 |
| GSM1083169 |
LCMV-Clone 13 spleen day 5, rep3 |
| GSM1083170 |
LCMV-Clone 13 spleen day 9, rep1 |
| GSM1083171 |
LCMV-Clone 13 spleen day 9, rep2 |
| GSM1083172 |
LCMV-Clone 13 spleen day 9, rep3 |
| GSM1083173 |
LCMV-Clone 13 spleen day 9, rep4 |
| GSM1083174 |
LCMV-Clone 13 spleen day 30, rep1 |
| GSM1083175 |
LCMV-Clone 13 spleen day 30, rep2 |
| GSM1083176 |
LCMV-Clone 13 spleen day 30, rep3 |
| GSM1083177 |
LCMV-Clone 13 spleen day 30, rep4 |
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| Relations |
| BioProject |
PRJNA189457 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE44322_RAW.tar |
126.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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