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| Status |
Public on Mar 16, 2013 |
| Title |
Enterococcus faecalis OG1RF control vs. treatment with ampicillin, bacitracin, or cephalothin |
| Platform organism |
Enterococcus faecalis |
| Sample organism |
Enterococcus faecalis OG1RF |
| Experiment type |
Expression profiling by array
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| Summary |
Transcriptional profiling to investigate the effect of drug treatment on the E. faecalis cells. For microarray analysis, E. faecalis OG1RF was grown in FMC medium supplemented with 10 mM glucose to an optical density at 600 nm (OD600) of 0.3 and the cultures were divided in 7 aliquots. One aliquot was collected by centrifugation and immediately frozen (untreated control cells). The other aliquots were treated for 30 or 60 min with 1.25 X the minimum inhibitory concentration (MIC) of one of the following CW inhibitors: ampicillin (20 μg ml-1), bacitracin (80 μg ml-1), or cephalothin (80 μg ml-1). After an exposure time of 30 or 60 minutes, each of these cultures was also centrifuged and the pellets frozen. RNA was then isolated from each pellet for microarray analysis. This process was repeated 3 additional times, for a total of four replicates of each condition.
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| Overall design |
RNA was extracted from four replicate samples of each condition of interest (control cells grown to OD600 = 0.3 in FMC medium supplemented with 10mM glucose, then treated with ampicillin, bacitracin, or cephalothin for 30 or 60 minutes) and labeled with Cy3. For each replicate, labeled RNA was hybridized to slides along with Cy5-labeled reference RNA, extracted from E. faecalis OG1RF cells grown in BHI medium to mid-log.
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| Contributor(s) |
Abranches J, Tijerina P, Avilés-Reyes A, Gaca AO, Kajfasz JK, Lemos J |
| Citation(s) |
23755154 |
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| Submission date |
Mar 15, 2013 |
| Last update date |
Jun 17, 2013 |
| Contact name |
Jessica K Kajfasz |
| E-mail(s) |
jkajfasz@dental.ufl.edu
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| Organization name |
University of Florida
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| Department |
Oral Biology
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| Lab |
Lemos-Abranches
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| Street address |
1395 Center Drive
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| City |
Gainesville |
| State/province |
FL |
| ZIP/Postal code |
32610 |
| Country |
USA |
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| Platforms (1) |
| GPL15039 |
JCVI PFGRC Enterococcus faecalis 14K v1 array (condensed) |
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| Samples (28)
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| GSM1099509 |
E. faecalis OG1RF control, replicate 4 |
| GSM1099510 |
E. faecalis OG1RF exposed to 20μg/mL ampicillin for 30 minutes, replicate 1 |
| GSM1099511 |
E. faecalis OG1RF exposed to 20μg/mL ampicillin for 30 minutes, replicate 2 |
| GSM1099512 |
E. faecalis OG1RF exposed to 20μg/mL ampicillin for 30 minutes, replicate 3 |
| GSM1099513 |
E. faecalis OG1RF exposed to 20μg/mL ampicillin for 30 minutes, replicate 4 |
| GSM1099514 |
E. faecalis OG1RF exposed to 20μg/mL ampicillin for 60 minutes, replicate 1 |
| GSM1099515 |
E. faecalis OG1RF exposed to 20μg/mL ampicillin for 60 minutes, replicate 2 |
| GSM1099516 |
E. faecalis OG1RF exposed to 20μg/mL ampicillin for 60 minutes, replicate 3 |
| GSM1099517 |
E. faecalis OG1RF exposed to 20μg/mL ampicillin for 60 minutes, replicate 4 |
| GSM1099518 |
E. faecalis OG1RF exposed to 80μg/mL bacitracin for 30 minutes, replicate 1 |
| GSM1099519 |
E. faecalis OG1RF exposed to 80μg/mL bacitracin for 30 minutes, replicate 2 |
| GSM1099520 |
E. faecalis OG1RF exposed to 80μg/mL bacitracin for 30 minutes, replicate 3 |
| GSM1099521 |
E. faecalis OG1RF exposed to 80μg/mL bacitracin for 30 minutes, replicate 4 |
| GSM1099522 |
E. faecalis OG1RF exposed to 80μg/mL bacitracin for 60 minutes, replicate 1 |
| GSM1099523 |
E. faecalis OG1RF exposed to 80μg/mL bacitracin for 60 minutes, replicate 2 |
| GSM1099524 |
E. faecalis OG1RF exposed to 80μg/mL bacitracin for 60 minutes, replicate 3 |
| GSM1099525 |
E. faecalis OG1RF exposed to 80μg/mL bacitracin for 60 minutes, replicate 4 |
| GSM1099526 |
E. faecalis OG1RF exposed to 80μg/mL cephalothin for 30 minutes, replicate 1 |
| GSM1099527 |
E. faecalis OG1RF exposed to 80μg/mL cephalothin for 30 minutes, replicate 2 |
| GSM1099528 |
E. faecalis OG1RF exposed to 80μg/mL cephalothin for 30 minutes, replicate 3 |
| GSM1099529 |
E. faecalis OG1RF exposed to 80μg/mL cephalothin for 30 minutes, replicate 4 |
| GSM1099530 |
E. faecalis OG1RF exposed to 80μg/mL cephalothin for 60 minutes, replicate 1 |
| GSM1099531 |
E. faecalis OG1RF exposed to 80μg/mL cephalothin for 60 minutes, replicate 2 |
| GSM1099532 |
E. faecalis OG1RF exposed to 80μg/mL cephalothin for 60 minutes, replicate 3 |
| GSM1099533 |
E. faecalis OG1RF exposed to 80μg/mL cephalothin for 60 minutes, replicate 4 |
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| This SubSeries is part of SuperSeries: |
| GSE45306 |
Enterococcus faecalis OG1RF control vs. treatment with ampicillin, bacitracin, cephalothin, or vancomycin |
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| Relations |
| BioProject |
PRJNA193278 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE45231_RAW.tar |
7.4 Mb |
(http)(custom) |
TAR (of MEV) |
| Processed data included within Sample table |
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