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Series GSE45793 Query DataSets for GSE45793
Status Public on Apr 11, 2013
Title Differential gene expression in cartilage from the mouse DMM osteoarthritis model
Organism Mus musculus
Experiment type Expression profiling by array
Summary Transcriptional profiling of mouse cartilage comparing destabilised medial meniscal operated knee joints to sham operated knee joints in both wild type C57Bl/6 and C57Bl/6 ADAMTS5Dcat mice at 1, 2 and 6 weeks post DMM surgery. The goal was to determine the differential gene expression with the onset of arthritis, both early, mid and late timepoints and to assess the gene expression differences when aggrecan loss is prevented in the ADAMTS5Dcat mouse.
 
Overall design The overall design of the experiment is to compare DMM (right leg) to sham operated (left leg) cartilage from the same mouse. In order to identify genes involved in early and late arthritis, RNA was collected from cartilage samples from mice at 1, 2 and 6 weeks post surgery. In addition, to isolate genes differentially expressed downstream of aggrecan loss, the RNA as described was collected from C57Bl/6 wild type mice and ADAMTS5Dcat mice. Four mice were used at each timepoint with their left leg being the control Sham and their right leg the experimental DMM. One sample from the wild type 1wk post operation and one ADAMTS5Dcat dye swap were removed from the analysis for technical reasons.
 
Contributor(s) Bateman J, Rowley L, Belluoccio D, Chan B, Bell K, Fosang A, Little C
Citation(s) 23436205, 23896777
Submission date Apr 04, 2013
Last update date Feb 04, 2020
Contact name Lynn Rowley
E-mail(s) lynn.rowley@mcri.edu.au
Organization name Murdoch Childrens Research Institute
Street address Flemington Road
City Parkville
State/province Vic
ZIP/Postal code 3435
Country Australia
 
Platforms (1)
GPL4134 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version)
Samples (37)
GSM1120018 Wild Type cartilage 1wk Post Surgery Biol Rep 1
GSM1120019 Wild Type cartilage 1wk Post Surgery Biol Rep 2
GSM1120020 Wild Type cartilage 1wk Post Surgery Biol Rep 3
Relations
BioProject PRJNA196707

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE45793_RAW.tar 591.7 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
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