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| Status |
Public on Sep 26, 2014 |
| Title |
Comparison between wild type E. coli and adhE (alcohol dehydrogenase) mutants, grown under anaerobic condition |
| Platform organisms |
Escherichia coli O157:H7 str. EDL933; Escherichia coli CFT073; Escherichia coli O157:H7 str. Sakai; Escherichia coli str. K-12 substr. MG1655 |
| Sample organisms |
Escherichia coli BL21(DE3); Escherichia coli BW25113 |
| Experiment type |
Expression profiling by array
|
| Summary |
To characterize anaerobic stress-induced expression in adhE mutants, the microarray experiment was performed with two serotypes of wild type E. coli and their adhE mutants: (1) K-12 strain, wild type; (2) B strain, wild type; (3) BW25113, adhE mutants; (4) BL21(DE3), adhE mutants.
Under anaerobic growth condition in glucose-containing complex medium, the wild type strains 1 and 2 grew well whereas the mutant strains 3 and 4 experienced anaerobic stress and grew after 24 hours and 48 hours, respectively.
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| Overall design |
For each of the four strains, RNA sampling was done after six hours of growth under anaerobic condition. Only one replicate was obtained for each strain.
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| Contributor(s) |
Lee S |
| Citation(s) |
25250024 |
| |
| Submission date |
Apr 29, 2013 |
| Last update date |
Sep 26, 2014 |
| Contact name |
Seungwoo Hwang |
| E-mail(s) |
swhwang10@yahoo.com
|
| Phone |
82-42-879-8544
|
| Organization name |
Korea Research Institute of Bioscience and Biotechnology
|
| Department |
Korean Bioinformation Center
|
| Street address |
52 Eoeun-dong Yuseong-gu
|
| City |
Daejeon |
| ZIP/Postal code |
305-806 |
| Country |
South Korea |
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| Platforms (1) |
| GPL13359 |
Agilent-020097 E. coli Gene Expression Microarray (Probe Name version) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA200557 |
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