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| Status |
Public on Feb 03, 2014 |
| Title |
Transcriptomic profiling of the development of the inflammatory response in human monocytes in vitro |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To investigate the time-dependent and coordinated sequence of inflammation-related events, and the dynamic features of macrophage polarisation/activation, we build and validated an in vitro model based on primary human monocytes
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| Overall design |
Monocytes were sequentially exposed to relevant stimuli: hrCCL2 (10 ng/ml; 0-2 h), LPS (5 ng/ml; 2-14 h) hrTNF-α (10 ng/ml; 3-14 h), hrIFN-γ (25 ng/ml; 7-14 h), hrIL-10 (20 ng/ml; 14-24 h), hrTGF-β (10 ng/ml; 24-48 h). The temperature was raised to 39 °C from 2 h to 14 h. Fresh monocytes were taken as time 0. Samples were collected at different time points and processed for total RNA extraction and hybridization on Affymetrix microarrays. Nine donors (A, B, C, D, E, F, G, H, L) were used for each of the 5 conditions (0, 4, 14, 24, 48 h) for a total of 45 samples, and 3 donors (M, N, O) were used for the following time-points: 0, 2, 2,5, 3, 3,5 h.
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| Contributor(s) |
Cifola I, Battaglia C, Italiani P, Boraschi D, Mazza EM, Bicciato S |
| Citation(s) |
24498352 |
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| Submission date |
May 21, 2013 |
| Last update date |
Feb 06, 2014 |
| Contact name |
Silvio Bicciato |
| E-mail(s) |
silvio.bicciato@unipd.it
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| Phone |
+39-049-827-6108
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| Organization name |
University of Padova
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| Department |
Molecular Medicine
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| Street address |
via U. Bassi 59/b
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| City |
Padova |
| ZIP/Postal code |
35131 |
| Country |
Italy |
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| Platforms (1) |
| GPL17180 |
Affymetrix GeneChip Human Genome U133 Array Set HG-U133A based on a custom CDF (GeneAnnot version 2.1.0) |
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| Samples (60)
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| GSM1145340 |
Donor D, time 0 |
| GSM1145341 |
Donor E, time 0 |
| GSM1145342 |
Donor F, time 0 |
| GSM1145343 |
Donor G, time 0 |
| GSM1145344 |
Donor H, time 0 |
| GSM1145345 |
Donor L, time 0 |
| GSM1145346 |
Donor M, time 0 |
| GSM1145347 |
Donor N, time 0 |
| GSM1145348 |
Donor O, time 0 |
| GSM1145349 |
Donor M, time 2 h |
| GSM1145350 |
Donor N, time 2 h |
| GSM1145351 |
Donor O, time 2 h |
| GSM1145352 |
Donor M, time 2.5 h |
| GSM1145353 |
Donor N, time 2.5 h |
| GSM1145354 |
Donor O, time 2.5 h |
| GSM1145355 |
Donor M, time 3 h |
| GSM1145356 |
Donor N, time 3 h |
| GSM1145357 |
Donor O, time 3 h |
| GSM1145358 |
Donor M, time 3.5 h |
| GSM1145359 |
Donor N, time 3.5 h |
| GSM1145360 |
Donor O, time 3.5 h |
| GSM1145361 |
Donor A, time 4 h |
| GSM1145362 |
Donor B, time 4 h |
| GSM1145363 |
Donor C, time 4 h |
| GSM1145364 |
Donor D, time 4 h |
| GSM1145365 |
Donor E, time 4 h |
| GSM1145366 |
Donor F, time 4 h |
| GSM1145367 |
Donor G, time 4 h |
| GSM1145368 |
Donor H, time 4 h |
| GSM1145369 |
Donor L, time 4 h |
| GSM1145370 |
Donor A, time 14 h |
| GSM1145371 |
Donor B, time 14 h |
| GSM1145372 |
Donor C, time 14 h |
| GSM1145373 |
Donor D, time 14 h |
| GSM1145374 |
Donor E, time 14 h |
| GSM1145375 |
Donor F, time 14 h |
| GSM1145376 |
Donor G, time 14 h |
| GSM1145377 |
Donor H, time 14 h |
| GSM1145378 |
Donor L, time 14 h |
| GSM1145379 |
Donor A, time 24 h |
| GSM1145380 |
Donor B, time 24 h |
| GSM1145381 |
Donor C, time 24 h |
| GSM1145382 |
Donor D, time 24 h |
| GSM1145383 |
Donor E, time 24 h |
| GSM1145384 |
Donor F, time 24 h |
| GSM1145385 |
Donor G, time 24 h |
| GSM1145386 |
Donor H, time 24 h |
| GSM1145387 |
Donor L, time 24 h |
| GSM1145388 |
Donor A, time 48 h |
| GSM1145389 |
Donor B, time 48 h |
| GSM1145390 |
Donor C, time 48 h |
| GSM1145391 |
Donor D, time 48 h |
| GSM1145392 |
Donor E, time 48 h |
| GSM1145393 |
Donor F, time 48 h |
| GSM1145394 |
Donor G, time 48 h |
| GSM1145395 |
Donor H, time 48 h |
| GSM1145396 |
Donor L, time 48 h |
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| Relations |
| BioProject |
PRJNA203689 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE47122_RAW.tar |
285.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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