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Status |
Public on Sep 01, 2006 |
Title |
FAIRE performed in human foreskin fibroblast cells |
Project |
Pilot ENCODE
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Organism |
Homo sapiens |
Experiment type |
Genome binding/occupancy profiling by genome tiling array
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Summary |
To perform FAIRE, chromatin is crosslinked with formaldehyde in vivo, sheared by sonication, and phenol-chloroform extracted. The DNA recovered in the aqueous phase is fluorescently labeled and hybridized to a DNA microarray. FAIRE performed in human cells strongly enriches DNA coincident with the location of DNaseI hypersensitive sites, transcriptional start sites, and active promoters. Keywords: Genome wide map of active chromatin
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Overall design |
Human foreskin fibroblast (ATCC CRL 2091) cells were cultured in 245 x 245 cm plates (90% confluent). Formaldehyde was added directly to the plates at room temperature to a final concentration of 1%; and incubated for 1, 2, 4, or 7 minutes. Cells were glass bead disrupted and sheared using sonication, yielding fragments of DNA 0.5 to 1 kb. Proteins and DNA fragments crosslinked to proteins were removed using a phenol/chloroform extraction.
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Web link |
http://www.ncbi.nlm.nih.gov/projects/geo/info/ENCODE.html
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Contributor(s) |
Giresi PG, Kim J, Iyer VR, Lieb JD |
Citation(s) |
17179217, 17571346 |
BioProject |
PRJNA63415 |
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Submission date |
May 22, 2006 |
Last update date |
Sep 14, 2012 |
Contact name |
Jason D Lieb |
E-mail(s) |
jlieb@bio.unc.edu
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Phone |
919-843-3229
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URL |
http://www.bio.unc.edu/faculty/lieb/labpages/default.shtml
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Organization name |
University of North Carolina at Chapel Hill
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Department |
Biology
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Lab |
Jason Lieb
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Street address |
203 Fordham Hall
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City |
Chapel Hill |
State/province |
NC |
ZIP/Postal code |
27599 |
Country |
USA |
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Platforms (1) |
GPL3514 |
ENCODE NimbleGen hg17_04252005 tiling array |
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Samples (4)
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GSM109841 |
FAIRE performed in human foreskin fibroblast cells crosslinked for 1 minute |
GSM109842 |
FAIRE performed in human foreskin fibroblast cells crosslinked for 2 minute |
GSM109843 |
FAIRE performed in human foreskin fibroblast cells crosslinked for 4 minutes |
GSM109844 |
FAIRE performed in human foreskin fibroblast cells crosslinked for 7 minutes |
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