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| Status |
Public on Sep 27, 2013 |
| Title |
Identification of microRNAs differentially expressed in prostatic secretions of patients with prostate cancer |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
In this study, we aimed to identify a miRNA expression signature that could be used to distinguish PCa from BPH. We have shown for the first time in the literature the presence of miRNAs in the PSS. We suggest PSS as a powerful non-invasive source for evaluation of prognosis in PCa, since prostate massages can be easily applied during routine examination. Our results showed that certain differentially expressed miRNAs in PSS could be used as diagnostics markers.
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| Overall design |
Prostate secretion samples (PSS) from 23 PCa and 25 benign prostate hyperplasia (BPH) patients were obtained from Urology Department of Bagcilar Educational and Research Hospital (Istanbul). MicroRNA (miRNA) profiling of eight PSS (four from BPH, four from PCa patients) were performed using microarray. Four of significantly deregulated miRNAs were further confirmed using quantitative reverse-transcription PCR (qRT-PCR). Statistical analysis was performed using Student’s t-test. ROC curves were plotted with SPSS-15.0.
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| Contributor(s) |
Guzel E, Karatas OF, Semercioz A, Ekici S, Aykan S, Yentur S, Creighton CJ, Mittmann M, Ozen M |
| Citation(s) |
24976077 |
| |
| Submission date |
Jul 29, 2013 |
| Last update date |
Oct 29, 2018 |
| Contact name |
Mustafa Ozen |
| E-mail(s) |
mozen@bcm.edu
|
| Organization name |
Istanbul University
|
| Department |
Medical Genetics
|
| Street address |
Fatih
|
| City |
Istanbul |
| ZIP/Postal code |
34300 |
| Country |
Turkey |
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| Platforms (1) |
| GPL15018 |
Agilent-031181 Unrestricted_Human_miRNA_V16.0_Microarray 030840 (Feature Number version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA213574 |
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