|
| Status |
Public on Sep 27, 2013 |
| Title |
Prostate Secretion Fluid_BPH1 |
| Sample type |
RNA |
| |
|
| Source name |
Prostate Secretion Fluid
|
| Organism |
Homo sapiens |
| Characteristics |
body fluid: Prostate Secretion Fluid
collection method: Prostate massages
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from equal amounts of secretion samples collected from BPH and PCa patients using ”mirVana PARIS kit” (Ambion, Darmstadt, Germany) according to the manufacturer’s protocol.
|
| Label |
Cy3
|
| Label protocol |
50 ng of total RNA from each sample are labeled with Cy3, via using “Agilent miRNA Labeling and Hyb Kit”.
|
| |
|
| Hybridization protocol |
Labeled RNAs are heat denatured and hybridized to Agilent 8x60K miRNA microarray V4 comprised of 1349 probes targeting a comprehensive selection of 1205 human and 144 virus miRNAs with control probes from Sanger miRBase (release 16) at 55o C for 20 hr.
|
| Scan protocol |
After hybridization and post-hybridization washes, the chip was scanned by Agilent Microarray Scanner with Surescan High Resolution Technology (Agilent Technologies, Santa Clara, CA).
|
| Data processing |
Scanned images were analyzed with Feature Extraction v10.7.3.1 (Agilent Technologies, CA) and Bioconductor software. Data are normalized by quantile normalization. P values and fold changes are calculated.
|
| |
|
| Submission date |
Jul 29, 2013 |
| Last update date |
Sep 28, 2013 |
| Contact name |
Mustafa Ozen |
| E-mail(s) |
mozen@bcm.edu
|
| Organization name |
Istanbul University
|
| Department |
Medical Genetics
|
| Street address |
Fatih
|
| City |
Istanbul |
| ZIP/Postal code |
34300 |
| Country |
Turkey |
| |
|
| Platform ID |
GPL15018 |
| Series (1) |
| GSE49298 |
Identification of microRNAs differentially expressed in prostatic secretions of patients with prostate cancer |
|
