|
| Status |
Public on Nov 29, 2013 |
| Title |
Evolution at two regulatory levels in yeast |
| Organisms |
Saccharomyces cerevisiae; Saccharomyces paradoxus; Saccharomyces cerevisiae x Saccharomyces paradoxus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Ribosome profiling performed on interspecific hybrids of Sacharromyces cerevisiae and S. paradoxus in order to identify allele-specific expression indicative of cis-regulatory divergence at the level of mRNA abundance and protein translation.
|
| |
|
| Overall design |
Two biological replicate libraries sequenced from ribosome protected fragments as well as poly-A-selected mRNA of hybrids in addition to one biological replicate library of poly-A selected mRNA from each parental strain.
|
| |
|
| Contributor(s) |
Artieri CG, Fraser HB |
| Citation(s) |
24318729 |
| BioProject |
PRJNA214730 |
| Submission date |
Aug 20, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Carlo G. Artieri |
| E-mail(s) |
carlo.artieri@gmail.com
|
| Phone |
650-723-1849
|
| Organization name |
Stanford University
|
| Department |
Biology
|
| Lab |
HB Fraser
|
| Street address |
Herrin Labs Rm. 309
|
| City |
Stanford |
| State/province |
California |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platforms (3) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
| GPL17601 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae x Saccharomyces paradoxus) |
| GPL17602 |
Illumina HiSeq 2000 (Saccharomyces paradoxus) |
|
| Samples (10)
|
| GSM1213147 |
Hybrid ribosome protected fragments replicate 1 |
| GSM1213148 |
Hybrid ribosome protected fragments replicate 2 |
| GSM1213149 |
Hybrid poly-A selected mRNA fragments replicate 1 |
| GSM1213150 |
Hybrid poly-A selected mRNA fragments replicate 2 |
| GSM1213151 |
S. cerevisiae poly-A selected mRNA fragments |
| GSM1213152 |
S. paradoxus poly-A selected mRNA fragments |
| GSM1278061 |
Mixed parental selected mRNA fragments |
| GSM1278062 |
Mixed parental ribosome protected fragments replicate 1 |
| GSM1278063 |
Mixed parental ribosome protected fragments replicate 2 lane 1 |
| GSM1278064 |
Mixed parental ribosome protected fragments replicate 2 lane 2 |
|
| Relations |
| SRA |
SRP028614 |
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