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| Status |
Public on Sep 30, 2013 |
| Title |
Male-specific Fruitless isoforms have different regulatory roles conferred by distinct zinc finger DNA binding domains |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Drosophila melanogaster adult males perform an elaborate courtship ritual to entice females to mate. fruitless (fru), a gene that is one of the key regulators of male courtship behavior, encodes multiple male-specific isoforms (FruM). These isoforms vary in their carboxy-terminal zinc finger domains, which are predicted to facilitate DNA binding. By over-expressing individual FruM isoforms in fru-expressing neurons in either males or females and assaying the global transcriptional response by RNA-sequencing, we show that three FruM isoforms have different regulatory activities that depend on the sex of the fly. We identified several sets of genes regulated downstream of FruM isoforms.
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| Overall design |
RNA seqeuncing was performed on mRNA derived from adult male or female heads, for a total of 39 samples. These samples included two wild type genotypes (Berlin and Canton-S), two transheterozygous mutants for fru P1 (Df(3R)P14/Df(3R)fru4-40 and fruw12/ Df(3R)ChaM5), and 3 overexpressing genotypes (fru P1-Gal4: UAS-FruMA, UAS-FruMB, UAS-FruMC). There were at least 3 replicates from biological samples for all sex by genotype combinations.
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| Contributor(s) |
Dalton JE, Fear JM, Knott S, Baker B, McIntyre LM, Arbeitman MN |
| Citation(s) |
24074028, 27172187 |
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| Submission date |
Aug 30, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Michelle N Arbeitman |
| E-mail(s) |
michelle.arbeitman@med.fsu.edu
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| Organization name |
Florida State University
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| Department |
Medicine Biomedical Sciences
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| Street address |
1115 W. Call Street
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| City |
Tallahassee |
| State/province |
Florida |
| ZIP/Postal code |
32306 |
| Country |
USA |
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| Platforms (1) |
| GPL11203 |
Illumina Genome Analyzer IIx (Drosophila melanogaster) |
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| Samples (39)
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| Relations |
| BioProject |
PRJNA220189 |
| SRA |
SRP030129 |
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