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| Status |
Public on Jul 16, 2003 |
| Title |
Fibroblast cells immediate-early after human cytomegalovirus infection |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by SAGE
|
| Summary |
Human cytomegalovirus (HCMV) has been shown to have the potential to alter cellular gene expression early after infection. However, one-gene approaches and the use of closed system gene expression technologies have identified only few cellular genes whose activity changed immediate-early. We therefore used serial analysis of gene expression (SAGE) to investigate the transcriptional program of human fibroblasts in response to HCMV in the immediate-early phase of infection. Differential expression of various cellular genes was monitored. Transcriptional expression changes of genes coding for ribosomal proteins reflected a general cellular response to starvation and stress. But differential regulation of genes coding for transcription factors and proteins associated with cellular metabolism, homeostasis and cell structure may represent transcriptional alterations in response to HCMV infection. Expression kinetics by 5' nuclease fluorigenic real-time PCR of selected genes revealed partial protection of infected cells against initial stress-associated alterations of gene expression and indicated fluctuations of transcriptional levels over time. Additionally, agreement with the quantitative results obtained by SAGE was observed only for genes up-regulated in HCMV-infected cells. This finding pointed to various technical and statistical parameters that all may be critical for quantitative transcriptome studies using global approaches, especially when exploring biological systems in a critical phase of cellular physiology.
Keywords: other
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| Contributor(s) |
Kenzelmann M, Muhlemann K |
| Citation(s) |
11124023 |
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| Submission date |
Jul 02, 2003 |
| Last update date |
Jun 27, 2016 |
| Contact name |
Marc Kenzelmann |
| E-mail(s) |
m.kenzelmann@dkfz.de
|
| Phone |
+41 31 632 3251
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| Fax |
+41 31 632 3550
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| Organization name |
Institute of Medical Microbiology, University of Bern
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| Department |
Molecular Genome Analysis
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| Street address |
Friedbuhlstrasse 51
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| City |
Bern |
| ZIP/Postal code |
3010 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL4 |
SAGE:10:NlaIII:Homo sapiens |
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| Samples (2) |
| GSM1 |
Foreskin Fibroblasts |
| GSM2 |
HCMV-infected foreskin fibroblasts |
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| Relations |
| BioProject |
PRJNA85223 |
