GEO Accession viewer

NCBI > GEO > Accession Display

Not logged in | Login

GEO help: Mouse over screen elements for information.

Sample GSM2

Query DataSets for GSM2

Status

Public on Sep 28, 2000

Title

HCMV-infected foreskin fibroblasts

Sample type

SAGE

Anchor

NlaIII

Tag Count

9497

Tag Length

Source name

mRNA of HCMV-infected human foreskin fibroblasts

Organism

Homo sapiens

Extracted molecule

total RNA

Description

Primary human foreskin fibroblasts at passages 15-18, cultured in Eagles Minimal Essential Medium (MEM) supplemented with penicillin and 10% fetal calf serum (FCS).
Confluent cells serum starved for 3 days with MEM containing 1% FCS.
85% of cells in G0/G1 cell cycle phase.
Cells infected at a multiplicity of infection (m.o.i.) of 5 with human cytomegalovirus (HCMV) strain AD169 infectious particles (virions) for 90 min at 37°Celsius.
mRNA isolated using a direct mRNA extraction kit.
Keywords = human cytomegalovirus, AD169, virions, HCMV-infected foreskin fibroblasts

Submission date

Sep 28, 2000

Last update date

Jun 27, 2016

Contact name

Marc Kenzelmann

E-mail(s)

m.kenzelmann@dkfz.de

Phone

+41 31 632 3251

Fax

+41 31 632 3550

Organization name

Institute of Medical Microbiology, University of Bern

Department

Molecular Genome Analysis

Street address

Friedbuhlstrasse 51

City

Bern

ZIP/Postal code

3010

Country

Switzerland

Platform ID

GPL4

Series (1)

GSE506

Fibroblast cells immediate-early after human cytomegalovirus infection

Data table header descriptions
TAG	Ten base SAGE tag,
COUNT	TAG NUMBER
TPM	tags per million

Data table
TAG	COUNT	TPM
AAAAAAAAAA	16	1684.74
AAAAAAAAAC	1	105.30
AAAAAAAAAG	1	105.30
AAAAAAAAAT	1	105.30
AAAAAAATTT	2	210.59
AAAAAACCCT	1	105.30
AAAAAAGCAG	1	105.30
AAAAAAGCTT	1	105.30
AAAAACCATA	1	105.30
AAAAACCCAT	1	105.30
AAAAACCCTT	1	105.30
AAAAACTGGA	1	105.30
AAAAAGCAGA	1	105.30
AAAAATAAAA	2	210.59
AAAAATAAAG	2	210.59
AAAAATATAA	1	105.30
AAAAATTTTC	1	105.30
AAAACAAAAT	1	105.30
AAAACAATGG	1	105.30
AAAACATCAT	1	105.30

Total number of rows: 5626

Table truncated, full table size 110 Kbytes.

Supplementary data files not provided

Processed data included within Sample table

Processed data are available on Series record