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| Status |
Public on Sep 13, 2013 |
| Title |
Human exosomal placenta-specific miRNAs modulate expression of target genes in maternal immune cells during pregnancy |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We investigated placenta-specific miRNA miR-517a (miR-517a-3p) functions in Jurkat cells. For this purpose, we identified candidate target mRNAs of the placenta-specific miRNAs using DNA microarray analysis. Transfection of Jurkat cells with miR-517a significantly downregulated 123 genes. Among the 123 genes identified, we searched for potential direct targets of miR-517a using the online software MicroCosm Targets. Seven genes, ALDH1B1, ANP32E, DHFR, FAT2, IGSF5, PRKG1, and RSPO3, had at least one potential miR-517a binding site in their 3’-UTRs. Furthermore, we revealed that PRKG1, one of the seven genes, was a miR-517a target using an in vitro experimental validation system.
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| Overall design |
We compared gene expression pattern of miR-517a–overexpressing Jurkat cells with that of negative control.
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| Contributor(s) |
Takizawa T |
| Citation(s) |
25273530 |
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| Submission date |
Sep 12, 2013 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Kazuya Yuge |
| E-mail(s) |
kyuge@nms.ac.jp
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| Organization name |
Nippon Medical School
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| Street address |
1-1-5
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| City |
Sendagi, Tokyo |
| ZIP/Postal code |
113-8602 |
| Country |
Japan |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA219070 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE50814_RAW.tar |
4.4 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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