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| Status |
Public on Nov 09, 2015 |
| Title |
ST3GAL1-Associated Transcriptomic Program in Glioblastoma Tumor Growth, Invasion, and Prognosis |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Cell surface sialylation confers many roles in cancer biology including cell proliferation, invasiveness, metastasis and angiogenesis. We show here that ST3Gal1 sialyltransferase marks a self-renewing cellular fraction. Depletion of ST3GAL1 abrogates glioma cell growth and tumorigenicity. In contrast, TGFb induces ST3GAL1 expression and correlates with the pattern of ST3Gal1 activation in patient tumors of the mesenchymal molecular subtype. To delineate the downstream events of ST3Gal1 signaling, we utilized a bioinformatical approach that leveraged on the greater statistical power of large patient databases, and subsequently verified our predictions in patient-derived glioma cells. We identify FoxM1, a major stem cell regulatory gene, as a downstream effector, and show that ST3Gal1 mediates the glioma phenotype through control of FoxM1 protein degradation
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| Overall design |
Total RNA from primary neurosphere culture of brain tumor specimens were flow-sorted to identify Peanut Agglutinin(PNA) affinity. The fractions of different degress of PNA bound GPCs were hybridized on Illumina Human Ref-8v2 bead chips to study the impact of transcriptome pattern of PNA affinity. Specimens were obtained from 4 patients and replicate arrays were performed for all 4 neurosphere cultures.
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| Contributor(s) |
Chong YK, Sandanaraj E, Chong YK, Sandanaraj E, Toh TB, Thangaveloo M, Tan MS, Koh LW, Lim GG, Holbrook JD, Kon OL, Nadarajah M, Ng I, Ng WH, Lim KL, Tang C, Ang BT |
| Citation(s) |
26547933 |
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| Submission date |
Oct 18, 2013 |
| Last update date |
Dec 11, 2015 |
| Contact name |
Edwin Sandanaraj |
| Phone |
6564070112
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| Organization name |
NNI
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| Department |
Research
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| Lab |
Neuro-Oncology
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| Street address |
11 Jalan Tan Tock Seng
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| City |
Singapore |
| ZIP/Postal code |
Singapore |
| Country |
Singapore |
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| Platforms (1) |
| GPL14186 |
Illumina HumanRef-8 v2 Expression Beadchip 1699538181 |
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| Samples (30)
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| This SubSeries is part of SuperSeries: |
| GSE51413 |
ST3GAL1-Associated Transcriptomic Program in Glioblastoma Tumor Growth, Invasion, and Prognosis |
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| Relations |
| BioProject |
PRJNA222988 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE51411_non-normalized.txt.gz |
2.5 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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