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| Status |
Public on Nov 11, 2013 |
| Title |
Expression data of the endothelial-to-hematopoietic transition in E8.5 concepti |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
During development a specialised subset of endothelial cells, the haemogenic endothelium, undergo an endothelial-to-haematopoietic transition. This process critically involves the transcription factor Runx1. Here we have isolated a specific subpopulation of endothelial cells using a Runx1 enhancer-reporter transgenic mouse line (23GFP). We have compared the gene expression profile of this population to non-23GFP expressing endothelial cells and CD41 expressing haematopoietic progenitor cells to assess whether 23GFP expression marks a biologically distinct subset of endothelium.
We used affymetrix microarrays to detail the global programme of gene expression underlying the different populations and identified a distinct expression profile of the 23GFP expressing endothelium.
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| Overall design |
23GFP transgenic mouse embryos were dissected free of the ectoplacental cone at embryonic day E8.5, pooled, and sorted into 23GFP- and 23GFP+ endothelial (VE-Cad+ Ter119- CD45- CD41-) and CD41+ haematopoietic cells (VE-Cad+ Ter119-CD45-CD41+23GFP+) cells by FACS. Triplicate samples for each cell population were analysed. cDNA synthesis, fragmentation, and hybridisation were performed by the Stanford Protein and Nucleic Acid Facility.
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| Contributor(s) |
de Bruijn M, Swiers G |
| Citation(s) |
24326267 |
| |
| Submission date |
Nov 04, 2013 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Stephen Taylor |
| E-mail(s) |
stephen.taylor@imm.ox.ac.uk
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| Phone |
+44 1865 222640
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| Organization name |
CBRG
|
| Street address |
Headington
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| City |
Oxford |
| ZIP/Postal code |
OX3 9DS |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA226619 |
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