|
| Status |
Public on Nov 11, 2013 |
| Title |
CD41+ HPC biological replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
VE-Cad+ Ter119- CD45- CD41+ 23GFP+ cells from E8.5 conceptus
|
| Organism |
Mus musculus |
| Characteristics |
genetic background: mixed (CBA x C57BL/6)
age: 4-9sp
cell type: VE-Cad+ Ter119- CD45- CD41+ 23GFP+ cells from E8.5 conceptus
|
| Treatment protocol |
Concepti were pooled and incubated for 20 mins at 37 degrees C in PBS with 10%FCS, 50U/ml penicillin and 50ug/ml streptomycin and 0.12% collagenase type I. A single cell suspension was generation by manual pipetting. Antibody staining was performed, and specific cell populations were isolated by FACS.
|
| Growth protocol |
E8.5 embryos were genotyped and staged by counting somite pairs.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA according to manufacturers instructions and purified using RNeasy Micro kit. Samples were amplified using WT-Ovation Pico RNA amplification system (NuGEN).
|
| Label |
biotin
|
| Label protocol |
Labelled and fragmented using WT Ovation cDNA Biotin Module v2 (NuGEN)
|
| |
|
| Hybridization protocol |
Hybridisation for 16 hr at 45C onto Affymetrix Mouse Genome 430 2.0 arrays by standard protocols at the Stanford Protein and Nucleic Acid facility
|
| Scan protocol |
Genechip Scanner 3000 7G
|
| Description |
Gene expression data for CD41 hematopoietic cells isolated from pooled E8.5 mouse concepti
|
| Data processing |
CEL files were normalised using RMA normalisation in the affy package.
|
| |
|
| Submission date |
Nov 04, 2013 |
| Last update date |
Nov 11, 2013 |
| Contact name |
Stephen Taylor |
| E-mail(s) |
stephen.taylor@imm.ox.ac.uk
|
| Phone |
+44 1865 222640
|
| Organization name |
CBRG
|
| Street address |
Headington
|
| City |
Oxford |
| ZIP/Postal code |
OX3 9DS |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE52075 |
Expression data of the endothelial-to-hematopoietic transition in E8.5 concepti |
|
