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Series GSE52316 Query DataSets for GSE52316
Status Public on Jul 08, 2014
Title VIB1, a link between glucose signaling and carbon catabolite repression, is essential for plant cell wall degradation by Neurospora crassa
Organism Neurospora crassa
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: To explore the function of VIB1 in regulating cellulase production in Neurospora crassa. Method: mRNA from vib-1 mutants were collected after a culture shift from sucrose to Avicel (crystaline cellulose) or carbon-free media. Expression profiles of vib-1 mutants were compared with published profiles of wild type created under the same conditions. Results: We found that many genes that specifically upregulated in wild type upon exposure to Avicel were expressed at low levels in ∆vib-1 and many other genes involved in metabolism and energy were expressed at high levels compared to wild type. Conclusions: Our study shows that VIB1 is required for suppression of glucose response and carbon catabolite repression to allow proper expression of clr-2 and subsequent cellulase production in response to cellulosic induction.
Overall design Biological triplicates of liquid culture N. crassa were harvested at 4 hours after a switch from sucrose media to media of interest. Global mRNA abundances were measured by sequencing on the Illumina Genome Analyzer Iix and HiSeq2000 platforms.
Contributor(s) Xiong Y, Sun J, Glass NL
Citation(s) 25144221
Submission date Nov 12, 2013
Last update date May 15, 2019
Contact name N. Louise Glass
Organization name UC Berkeley
Department Plant and Microbial Biology
Lab Glass
Street address 341 Koshland Hall, Univerisity of California
City Berkeley
State/province CA
ZIP/Postal code 94720-3102
Country USA
Platforms (2)
GPL16164 Illumina HiSeq 2000 (Neurospora crassa)
GPL17918 Illumina Genome Analyzer IIx (Neurospora crassa)
Samples (9)
GSM1262788 ∆vib-1 No Carbon #1
GSM1262789 ∆vib-1 No Carbon #2
GSM1262790 ∆vib-1 No Carbon #3
BioProject PRJNA227384
SRA SRP032954

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Supplementary file Size Download File type/resource
GSE52316_RAW.tar 2.3 Mb (http)(custom) TAR (of FPKM_TRACKING)
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Raw data are available in SRA
Processed data provided as supplementary file

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