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| Status |
Public on Jul 08, 2014 |
| Title |
VIB1, a link between glucose signaling and carbon catabolite repression, is essential for plant cell wall degradation by Neurospora crassa |
| Organism |
Neurospora crassa |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: To explore the function of VIB1 in regulating cellulase production in Neurospora crassa. Method: mRNA from vib-1 mutants were collected after a culture shift from sucrose to Avicel (crystaline cellulose) or carbon-free media. Expression profiles of vib-1 mutants were compared with published profiles of wild type created under the same conditions. Results: We found that many genes that specifically upregulated in wild type upon exposure to Avicel were expressed at low levels in ∆vib-1 and many other genes involved in metabolism and energy were expressed at high levels compared to wild type. Conclusions: Our study shows that VIB1 is required for suppression of glucose response and carbon catabolite repression to allow proper expression of clr-2 and subsequent cellulase production in response to cellulosic induction.
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| Overall design |
Biological triplicates of liquid culture N. crassa were harvested at 4 hours after a switch from sucrose media to media of interest. Global mRNA abundances were measured by sequencing on the Illumina Genome Analyzer Iix and HiSeq2000 platforms.
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| Contributor(s) |
Xiong Y, Sun J, Glass NL |
| Citation(s) |
25144221 |
| |
| Submission date |
Nov 12, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
N. Louise Glass |
| E-mail(s) |
lglass@berkeley.edu
|
| Organization name |
UC Berkeley
|
| Department |
Plant and Microbial Biology
|
| Lab |
Glass
|
| Street address |
341 Koshland Hall, Univerisity of California
|
| City |
Berkeley |
| State/province |
CA |
| ZIP/Postal code |
94720-3102 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL16164 |
Illumina HiSeq 2000 (Neurospora crassa) |
| GPL17918 |
Illumina Genome Analyzer IIx (Neurospora crassa) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA227384 |
| SRA |
SRP032954 |
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