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| Status |
Public on Dec 10, 2013 |
| Title |
Expression profile of E. coli in response to exogenous addition of isopentenol |
| Platform organism |
Escherichia coli K-12 |
| Sample organism |
Escherichia coli |
| Experiment type |
Expression profiling by array
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| Summary |
Exogenous isopentenol was added to a culture of E. coli and the RNA expression response was measured using Nimblegen arrays. Genes highly upregulated were then subsequently overexpressed to improve the tolerance to isopentenol toxicity. In addition a subset of genes that improved the tolerance were also overexpressed in a producing strain, leading to improved production over the empty vector control.
This work is described in Foo et al, Improving microbial bio-gasoline production in E. coli using tolerance engineering (in preparation)
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| Overall design |
Six independent biological replicates were grown in M9 medium. 0.2% v/v isopentenol was added to 3 cultures while 3 cultures received no treatment, and 2.5 hr later total RNA was extracted. One sample from each culture was hybridized.
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| Contributor(s) |
Foo JL, Jensen HM, Dahl RH, George K, Keasling JD, Lee TS, Leong S, Mukhopadhyay A |
| Citation(s) |
25370492 |
| |
| Submission date |
Dec 09, 2013 |
| Last update date |
Jul 31, 2018 |
| Contact name |
Robert Dahl |
| E-mail(s) |
robdahl@berkeley.edu
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| Organization name |
University of California-Berkeley
|
| Department |
Chemical Engineering
|
| Lab |
Keasling
|
| Street address |
5885 Hollis St, JBEI
|
| City |
Emeryville |
| State/province |
CA |
| ZIP/Postal code |
94608 |
| Country |
USA |
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| Platforms (1) |
| GPL14649 |
NimbleGen E. coli K12 Gene Expression Array [071112_Ecoli_K12_EXP] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA230972 |
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