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Status |
Public on Jul 21, 2014 |
Title |
Gene Expression Profiling of Peri-implant Healing of PLGA-Li+ Implants Suggests an Activated Wnt Signaling Pathway in vivo |
Organism |
Rattus norvegicus |
Experiment type |
Expression profiling by array
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Summary |
Bone development and regeneration is associated with the Wnt signaling pathway that, according to literature, can be modulated by lithium ions (Li+). The aim of this study was to evaluate the gene expression profile during peri-implant healing of poly(lactic-co-glycolic acid) (PLGA) implants with incorporated Li+, while PLGA without Li+ was used as control, and a special attention was then paid to the Wnt signaling pathway. The implants were inserted in rat tibia for 7 or 28 days and the gene expression profile was investigated using a genome-wide microarray analysis. The results were verified by qPCR and immunohistochemistry. Histomorphometry was used to evaluate the possible effect of Li+ on bone regeneration. The microarray analysis revealed a large number of significantly differentially regulated genes over time within the two implant groups. The Wnt signaling pathway was significantly affected by Li+, with approximately 34% of all Wnt-related markers regulated over time, compared to 22% for non-Li+ containing (control; Ctrl) implants. Functional cluster analysis indicated skeletal system morphogenesis, cartilage development and condensation as related to Li+. The downstream Wnt target gene, FOSL1, and the extracellular protein-encoding gene, ASPN, were significantly upregulated by Li+ compared with Ctrl. The presence of β-catenin, FOSL1 and ASPN positive cells was confirmed around implants of both groups. Interestingly, a significantly reduced bone area was observed over time around both implant groups. The presence of periostin and calcitonin receptor-positive cells was observed at both time points. This study is to the best of the authors’ knowledge the first report evaluating the effect of a local release of Li+ from PLGA at the fracture site. The present study shows that during the current time frame and with the present dose of Li+ in PLGA implants, Li+ is not an enhancer of early bone growth, although it affects the Wnt signaling pathway.
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Overall design |
PLGA implants with +/- incorporated Li were inserted in rat tibia for 7 or 28 days, peri-implant bone was harvested and RNA extracted using Qiazol lysis reagent and TissueLyser followed by Qiagen's Rneasy Micro Kit. The microarray experiment was conducted at SCIBLU Genomics (www.lu.se/sciblu) according to Affymetrix guidelines (n=6).
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Contributor(s) |
Thorfve A, Tengvall P |
Citation(s) |
25047349 |
Submission date |
Jan 22, 2014 |
Last update date |
Feb 07, 2019 |
Contact name |
Anna Thorfve |
E-mail(s) |
anna.thorfve@biomaterials.gu.se
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Phone |
0046 31 786 29 19
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Organization name |
University of Gothenburg
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Department |
Biomaterials
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Lab |
Box 412
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Street address |
Arvid Wallgrens backe 20
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City |
Gothenburg |
ZIP/Postal code |
SE 405 30 |
Country |
Sweden |
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Platforms (1) |
GPL17117 |
[RaGene-2_0-st] Affymetrix Rat Gene 2.0 ST Array [transcript (gene) version] |
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Samples (24)
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Relations |
BioProject |
PRJNA236109 |
Supplementary file |
Size |
Download |
File type/resource |
GSE54294_RAW.tar |
240.7 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
Processed data included within Sample table |
Processed data provided as supplementary file |
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