|
| Status |
Public on Jul 21, 2014 |
| Title |
Li day 28 rat 29 |
| Sample type |
RNA |
| |
|
| Source name |
Li-PLGA day 28
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague Dawley
gender: male
treatment group: poly(lactide-co-glycolide acid) (PLGA) implants with incorporated Li+
tissue: peri-implant bone (tibia)
time point: day 28
|
| Treatment protocol |
Rat tibia fracture model, Li-PLGA or Ctrl-PLGA implants, 7 or 28 days
|
| Growth protocol |
The animals were allowed free postoperative movement, with food and water ad libitum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen's Qizaol, TissueLyser and Qiagen Rneasy Micro kit
|
| Label |
Biotin Allonamide Triphosphate
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip rat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
|
| Description |
29_B
|
| Data processing |
The data were analyzed using expression console software version 1.1.2 using default analysis settings and RMA as normalization method.
|
| |
|
| Submission date |
Jan 22, 2014 |
| Last update date |
Jul 21, 2014 |
| Contact name |
Anna Thorfve |
| E-mail(s) |
anna.thorfve@biomaterials.gu.se
|
| Phone |
0046 31 786 29 19
|
| Organization name |
University of Gothenburg
|
| Department |
Biomaterials
|
| Lab |
Box 412
|
| Street address |
Arvid Wallgrens backe 20
|
| City |
Gothenburg |
| ZIP/Postal code |
SE 405 30 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL17117 |
| Series (1) |
| GSE54294 |
Gene Expression Profiling of Peri-implant Healing of PLGA-Li+ Implants Suggests an Activated Wnt Signaling Pathway in vivo |
|
