|
| Status |
Public on Feb 28, 2014 |
| Title |
Feedback control of Set1 protein levels is important for proper H3K4 methylation patterns |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome binding/occupancy profiling by array
|
| Summary |
Methylation of histone H3 lysine 4 by the Set1 subunit of COMPASS correlates withactive transcription. Here we show that Set1 levels are regulated by protein degradation in response to multiple signals. Set1 levels are greatly reduced when COMPASS recruitment to genes, H3K4 methylation, or transcription is blocked. The degradation sequences map to N-terminal regions that overlap a previously identified auto-inhibitory domain, as well as the catalytic domain. Truncation mutants of Set1 that cause under- or over-expression produce abnormal H3K4 methylation patterns on transcribed genes. Surprisingly, SAGA-dependent genes are more strongly affected than TFIID-dependent genes, reflecting differences in their chromatin dynamics. We propose that careful tuning of Set1 levels by regulated degradation is critical for establishment and maintenance of proper H3K4 methylation patterns.
|
| |
|
| Overall design |
Genome binding/occupancy profiling of H3K4me2 and H3K4me3 in yeast
|
| |
|
| Contributor(s) |
Soares LM, Radman-Livaja M, Lin SG, Rando OJ, Buratowski S |
| Citation(s) |
24613354 |
| |
| Submission date |
Feb 17, 2014 |
| Last update date |
Feb 15, 2018 |
| Contact name |
Marta Radman-Livaja |
| E-mail(s) |
mrl5374@gmail.com
|
| Phone |
+33434359667
|
| Organization name |
CNRS
|
| Department |
IGMM
|
| Street address |
1919 route de Mende
|
| City |
Montpellier |
| ZIP/Postal code |
34293 |
| Country |
France |
| |
|
| Platforms (1) |
| GPL4131 |
Agilent-014810 Yeast Whole Genome ChIP-on-Chip Microarray 4x44K (G4493A) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA238478 |
Less...
More...