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| Status |
Public on Jun 10, 2014 |
| Title |
MicroRNA-223 and miR-143 are important systemic biomarkers for disease activity in psoriasis |
| Platform organisms |
Homo sapiens; Mus musculus; Rattus norvegicus |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Background: Psoriasis is a systemic inflammatory skin disease. MicroRNAs (miRNAs) are a class of small non-coding RNA molecules that recently have been found in the blood to be relevant as disease biomarkers. Objective: We aimed to explore miRNAs potential as blood biomarkers for psoriasis. Methods: Using microarray and quantitative real-time PCR we measured the global miRNA expression in whole blood, plasma and peripheral blood mononuclear cells (PBMCs) from patients with psoriasis and healthy controls. Results: We identified several deregulated miRNAs in the blood from patients with psoriasis including miR-223 and miR-143 which were found to be significantly upregulated in the PBMCs from patients with psoriasis compared with healthy controls (FCH=1.63, P<0.01; FCH=2.18, P<0.01, respectively). In addition, miR-223 and miR-143 significantly correlated with the PASI score (r = 0.46, P<0.05; r=0.55, P<0.02, respectively). Receiver-operating characteristic analysis (ROC) showed that miR-223 and -143 have the potential to distinguish between psoriasis and healthy controls (miR-223: Area under the curve (AUC) = 0.80, miR-143: AUC = 0.75). Interestingly, after 3-5 weeks of treatment with methotrexate following a significant decrease in psoriasis severity, miR-223 and miR-143 were significantly downregulated in the PBMCs from patients with psoriasis. Conclusion: We suggest that changes in the miR-223 and miR-143 expressions in PBMCs from patients with psoriasis may serve as novel biomarkers for disease activity in psoriasis; however, further investigations are warranted to clarify their specific roles.
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| Overall design |
In the present study, we compared the global miRNA expression profile between whole blood samples obtained from 24 patients with psoriasis (5 samples were excluded due to poor quality control) compared with 15 healthy controls.
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| Contributor(s) |
Løvendorf MB, Zibert JR, Gyldenløve M, Røpke MA, Skov L |
| Citation(s) |
24909097 |
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| Submission date |
Mar 03, 2014 |
| Last update date |
Jun 10, 2014 |
| Contact name |
Marianne Bengtson Løvendorf |
| E-mail(s) |
mabelo01@geh.regionh.dk
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| Organization name |
Gentofte Hospital
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| Department |
Dermato-Allergology
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| Street address |
Niels Andersens Vej 65
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| City |
Hellerup |
| ZIP/Postal code |
2900 |
| Country |
Denmark |
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| Platforms (1) |
| GPL11241 |
miRCURY LNA microRNA Array, 5th generation - hsa, mmu & rno |
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| Samples (34)
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| Relations |
| BioProject |
PRJNA239819 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE55515.txt.gz |
138.0 Kb |
(ftp)(http) |
TXT |
| GSE55515_RAW.tar |
29.7 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data are available on Series record |
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