|
| Status |
Public on Jun 10, 2014 |
| Title |
PAX_healthy_011 |
| Sample type |
RNA |
| |
|
| Source name |
Whole blood
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Whole blood
disease: Healthy Control
age: 22
gender: M
ethnicity: Caucasian
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the PAXgene blood miRNA kit (PreAnalytiX/Qiagen, Copenhagen, Denmark) following the manufacturer’s instructions. The quality of the total RNA was verified by an Agilent 2100 Bioanalyzer profile.
|
| Label |
Hy3
|
| Label protocol |
500 ng total RNA from sample was labelled with Hy3™ fluorescent label using the miRCURY™ LNA Array power labelling kit (Exiqon, Denmark) following the procedure described by the manufacturer.
|
| |
|
| Hybridization protocol |
The Hy3™-labeled samples was hybridized to the miRCURY™ LNA Array version 5th Generation (Exiqon, Denmark), which contains capture probes targeting all miRNAs for human, mouse or rat registered in the miRBASE version 15.0 at the Sanger Institute. The hybridization was performed according to the miRCURY™ LNA array manual using a Tecan HS4800 hybridization station (Tecan, Austria).
|
| Scan protocol |
The miRCURY™ LNA array microarray slides were scanned using the Agilent G2565BA Microarray Scanner System (Agilent Technologies, Inc., USA) and the image analysis was carried out using the ImaGene 9.0 software (BioDiscovery, Inc., USA).
|
| Data processing |
The quantified signals were background corrected (Normexp with offset value 10 – Ritchie et al., Bioinformatics; 23: 2700-2707, 2007) and normalized using the Quantile algorithm.
|
| |
|
| Submission date |
Mar 03, 2014 |
| Last update date |
Jun 10, 2014 |
| Contact name |
Marianne Bengtson Løvendorf |
| E-mail(s) |
mabelo01@geh.regionh.dk
|
| Organization name |
Gentofte Hospital
|
| Department |
Dermato-Allergology
|
| Street address |
Niels Andersens Vej 65
|
| City |
Hellerup |
| ZIP/Postal code |
2900 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL11241 |
| Series (1) |
| GSE55515 |
MicroRNA-223 and miR-143 are important systemic biomarkers for disease activity in psoriasis |
|
