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| Status |
Public on Jun 13, 2014 |
| Title |
Gene expression profiling of breast fibroadenomas |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Fibroadenomas are the most common benign breast tumors in women under 30. Unlike their malignant counterparts, relatively molecular profiling has been done on fibroadenomas. Here we performed gene expression profiling on ten fibroadenomas in order to better characterize these tumors. Through targeted amplicon sequencing, we have found that six of these tumors have MED12 mutations. We show that the MED12 mutations, among others, are associated with activated estrogen signaling, as well as increased invasiveness through upregulation of ECM remodelling genes.
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| Overall design |
Microarray gene expression profiling was performed on 10 breast fibroadenomas and a combination of unsupervised and supervised clustering was performed to determine any differentially activated pathways between samples with mutated and non-mutated MED12.
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| Contributor(s) |
Lim WK, Tan J, Ong CK, Teh BT |
| Citation(s) |
25038752 |
| |
| Submission date |
Mar 05, 2014 |
| Last update date |
Sep 12, 2014 |
| Contact name |
Weng Khong Lim |
| E-mail(s) |
wengkhong.lim@duke-nus.edu.sg
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| Organization name |
National Cancer Centre Singapore
|
| Department |
Department of Medical Sciences
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| Lab |
NCCS-VARI Translational Research Laboratory
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| Street address |
11 Hospital Drive
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| City |
Singapore |
| State/province |
Singapore |
| ZIP/Postal code |
169610 |
| Country |
Singapore |
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| Platforms (1) |
| GPL17811 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array [CDF: Brainarray HGU133Plus2_Hs_ENTREZG_v17] |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA240194 |
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