|
| Status |
Public on Jun 13, 2014 |
| Title |
Fibroadenoma sample 002 |
| Sample type |
RNA |
| |
|
| Source name |
Excised fibroadenoma tumor
|
| Organism |
Homo sapiens |
| Characteristics |
med12 status: Mutant
age: 21
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to manufacturer's instructions, followed by purification using the Qiagen RNAeasy kit.
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol, 10ug of RNAeasy-purified total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 7G
|
| Description |
Gene expression profiling of breast fibroadenomas
|
| Data processing |
CEL files were loaded into R and then preprocessed and normalized using RMA. A custom CDF was used to reannotate the probes (http://brainarray.mbni.med.umich.edu/Brainarray/Database/CustomCDF/17.0.0/entrezg.asp).
|
| |
|
| Submission date |
Mar 05, 2014 |
| Last update date |
Jun 13, 2014 |
| Contact name |
Weng Khong Lim |
| E-mail(s) |
wengkhong.lim@duke-nus.edu.sg
|
| Organization name |
National Cancer Centre Singapore
|
| Department |
Department of Medical Sciences
|
| Lab |
NCCS-VARI Translational Research Laboratory
|
| Street address |
11 Hospital Drive
|
| City |
Singapore |
| State/province |
Singapore |
| ZIP/Postal code |
169610 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL17811 |
| Series (1) |
| GSE55594 |
Gene expression profiling of breast fibroadenomas |
|
